Two-dimensional glass/p-ATP/Ag NPs as multifunctional SERS substrates for label-free quantification of uric acid in sweat

Dechan Lu; Rongyuan Cai; Yuqin Liao; Ruiyun You; Yudong Lu

doi:10.1016/j.saa.2023.122631

Two-dimensional glass/p-ATP/Ag NPs as multifunctional SERS substrates for label-free quantification of uric acid in sweat

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Aug 5:296:122631. doi: 10.1016/j.saa.2023.122631. Epub 2023 Mar 22.

Authors

Dechan Lu¹, Rongyuan Cai², Yuqin Liao³, Ruiyun You⁴, Yudong Lu⁵

Affiliations

¹ College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, Fujian 350007, China; College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, Fujian 350007, China.
² College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, Fujian 350007, China; Shaanxi Science and Technology Exchange Center, Shaanxi 710054, China.
³ College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, Fujian 350007, China.
⁴ College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, Fujian 350007, China. Electronic address: youruiyun@fjnu.edu.cn.
⁵ College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, Fujian 350007, China. Electronic address: luyd@fjnu.edu.cn.

PMID: 37037174
DOI: 10.1016/j.saa.2023.122631

Abstract

Abnormal uric acid (UA) content in body fluids can fully reflect the status of metabolism and immunity in the body. We have developed a simple, efficient and label-free surface enhanced Raman scattering (SERS) method for UA detection. Briefly, p-aminothiophenol (p-ATP) was used as the internal standard molecule and linking molecule to prepare a glass/p-ATP/Ag NPs SERS substrate. The Raman characteristic peak of p-ATP at 1076 cm^-1 can be used as an internal standard molecule to correct the signal fluctuation of UA detection. The results show that the SERS method owns a linear response with a ranging from 5 × 10^-6 to 10^-3 M of UA characteristic peak of both 693 cm^-1 and 493 cm^-1 with a determination coefficient (R²) of 0.9878 and 0.9649, respectively. Additionally, the SERS sensor has been further used for the analysis of UA in sweat and good recoveries were obtained for the sensing of sweat. We believe that the developed SERS substrate has potential for applications in healthcare monitoring.

Keywords: Internal standard; SERS; Sweat; Uric acid.

MeSH terms

Adenosine Triphosphate
Metal Nanoparticles* / chemistry
Silver / chemistry
Spectrum Analysis, Raman / methods
Sweat*
Uric Acid

Substances

Uric Acid
Silver
4-aminothiophenol
Adenosine Triphosphate