Hyaluronidase-inhibiting Polysaccharide from Caulerpa lentillifera

Mahanama Geegana Gamage Awanthi; Saki Nagamoto; Hirosuke Oku; Kanefumi Kitahara; Teruko Konishi

doi:10.5458/jag.jag.JAG-2022_0004

Hyaluronidase-inhibiting Polysaccharide from Caulerpa lentillifera

J Appl Glycosci (1999). 2023 Mar 2;70(1):1-7. doi: 10.5458/jag.jag.JAG-2022_0004. eCollection 2023.

Authors

Mahanama Geegana Gamage Awanthi¹, Saki Nagamoto², Hirosuke Oku^{1

3}, Kanefumi Kitahara^{1

4}, Teruko Konishi^{1

2}

Affiliations

¹ 1 The United Graduate School of Agricultural Sciences, Kagoshima University.
² 2 Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus.
³ 3 Tropical Biosphere Research Center, University of the Ryukyus.
⁴ 4 Department of Food Science and Biotechnology, Faculty of Agriculture, Kagoshima University.

Abstract

Algal sulfated polysaccharides are known to be effective hyaluronidase inhibitors. We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera. Results showed that SP with IC₅₀ of 163 µg/mL appears to allosterically inhibit the hyaluronidase activity. Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7. We modified the SP by desulfation and partial hydrolysis with trifluoroacetic acid (TFA) to investigate the effect of sulfate content and molecular weight on inhibition. Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.

Keywords: Caulerpa lentillifera; algal polysaccharides; hyaluronidase inhibition; sulfated polysaccharide.

2023 by The Japanese Society of Applied Glycoscience.