Impact of tooth mineral tissues genes on dental caries: A birth-cohort study

Luiz Alexandre Chisini; Francine da Costa Santos; Rodrigo Varella de Carvalho; Bernardo Lessa Horta; Luciana Tovo-Rodrigues; Flávio Fernando Demarco; Marcos Britto Correa

doi:10.1016/j.jdent.2023.104505

Impact of tooth mineral tissues genes on dental caries: A birth-cohort study

J Dent. 2023 Jun:133:104505. doi: 10.1016/j.jdent.2023.104505. Epub 2023 Apr 7.

Authors

Luiz Alexandre Chisini¹, Francine da Costa Santos², Rodrigo Varella de Carvalho³, Bernardo Lessa Horta², Luciana Tovo-Rodrigues², Flávio Fernando Demarco¹, Marcos Britto Correa⁴

Affiliations

¹ Federal University of Pelotas, Address: 457, Gonçalves Chaves St. 5th floor, Pelotas, RS 96015-560, Brazil.
² Federal University Pelotas, Address: 1160, Marechal Deodoro St. Pelotas, RS 96020-220, Brazil.
³ Federal University of Juiz de Fora, Address: 745, São Paulo St., Governador Valadares, MG 35010-180, Brazil.
⁴ Federal University of Pelotas, Address: 457, Gonçalves Chaves St. 5th floor, Pelotas, RS 96015-560, Brazil. Electronic address: marcos.britto@ufpel.edu.br.

PMID: 37031884
DOI: 10.1016/j.jdent.2023.104505

Abstract

Objective: We aimed to investigate whether Single Nucleotide Polymorphisms present in the genes of tooth mineral tissues influence dental caries trajectory across the life course, and if there is an epistatic (gene-gene) interaction between these SNPs.

Methods: A representative sample of all 5,914 births from the 1982 Pelotas birth cohort study was prospectively investigated. Dental caries trajectory across the life course was assessed at 15(n = 888), 24(n = 720), and 31 years old(n = 539). Group-based trajectory modeling was used to identify distinct subgroups of individuals whose caries measurements followed a similar pattern over time. Genetic material was collected, and individuals were genotyped [rs4970957(TUFT1), rs1711437(MMP20), rs1784418(MMP20), rs2252070(MMP13), rs243847(MMP2), rs2303466(DLX3), rs11656951(DLX3), rs7501477(TIMP2), rs388286(BMP7), and rs5997096(TFIP11)]. Analyzes were performed for allele and genotype using logistic regression and generalized multifactor dimensionality reduction for epistatic interactions.

Results: The analyses included 678 individuals, those with allele C (OR=0.74, CI95%[0.59-0.92]), genotype CC in the additive effect (OR=0.52, CI95%[0.31-0.89]), and the genotype TC/CC in dominant effect (OR=0.72, CI95%[0.53-0.98]) on the rs243847(MMP2) were associated with low caries trajectory. Individuals with the allele T (OR=0.79, CI95%[0.64-0.98]) and the genotype TC/CC in dominant effect (OR=0.66, CI95%[0.47-0.95]) on the rs5997096(TFIP11) were associated with low caries trajectory. Positive epistatic interactions were observed involving two (MMP2 and BMP7; p = 0.006) and three (TUFT1, MMP2, and TFIP11; p<0.001) loci and high caries trajectory.

Conclusions: Some SNPs present in the genes of tooth mineral tissues were associated with caries trajectory and epistatic interactions increasing the network of SNPs involved in individual caries experience.

Clinical significance: Single nucleotide polymorphisms in the pathway of tooth mineral tissues genes may contribute significantly to the individual caries experience across the life course.

Keywords: Dental caries; Gene; Genetic; Polymorphisms; Single nucleotide polymorphisms; Tooth mineral genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cohort Studies
Dental Caries* / epidemiology
Dental Caries* / genetics
Humans
Matrix Metalloproteinase 2*
Matrix Metalloproteinase 20
Minerals

Substances

Matrix Metalloproteinase 2
Matrix Metalloproteinase 20
Minerals