Growth of spiral ganglion neurons induced by graphene oxide/oxidized bacterial cellulose composite hydrogel

Lin Shi; Guodong Hong; Chuntao Chen; Xueqian Li; Heng Zhang; Renjie Chai; Dongping Sun

doi:10.1016/j.carbpol.2023.120749

Growth of spiral ganglion neurons induced by graphene oxide/oxidized bacterial cellulose composite hydrogel

Carbohydr Polym. 2023 Jul 1:311:120749. doi: 10.1016/j.carbpol.2023.120749. Epub 2023 Mar 1.

Authors

Lin Shi¹, Guodong Hong², Chuntao Chen³, Xueqian Li¹, Heng Zhang¹, Renjie Chai⁴, Dongping Sun⁵

Affiliations

¹ Institute of Chemicobiology and Functional Materials, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, 200 Xiao Ling Wei Street, Nanjing 210094, Jiangsu Province, China.
² State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing 210096, China.
³ Institute of Chemicobiology and Functional Materials, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, 200 Xiao Ling Wei Street, Nanjing 210094, Jiangsu Province, China. Electronic address: chchunt@njust.edu.cn.
⁴ State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing 210096, China; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. Electronic address: renjiec@seu.edu.cn.
⁵ Institute of Chemicobiology and Functional Materials, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, 200 Xiao Ling Wei Street, Nanjing 210094, Jiangsu Province, China. Electronic address: sundpe301@163.com.

PMID: 37028876
DOI: 10.1016/j.carbpol.2023.120749

Abstract

The damage or degeneration of spiral ganglion neurons (SGNs) can impair the auditory signals transduction from hair cells to the central auditory system, and cause significant hearing loss. Herein, a new form of bioactive hydrogel incorporating topological graphene oxide (GO) and TEMPO-oxidized bacterial cellulose (GO/TOBC hydrogel) was developed to provide a favorable microenvironment for SGN neurite outgrowth. As the network structure of lamellar interspersed fiber cross-linked by GO/TOBC hydrogels well simulated the structure and morphology of ECM, with the controllable hydrophilic property and appropriate Young's modulus well met those requirements of SGNs microenvironment, the GO/TOBC hybrid matrix exhibited great potential to promote the growth of SGNs. The quantitative real-time PCR result confirmed that the GO/TOBC hydrogel can significantly accelerate the development of growth cones and filopodia, by increasing the mRNA expression levels of diap3, fscn2, and integrin β1. These results suggest that GO/TOBC hydrogel scaffolds have the potential to be used to construct biomimetic nerve grafts for repairing or replacing nerve defects.

Keywords: Cell growth; Graphene oxide; Hydrogel; Oxidized bacterial cellulose; Spiral ganglion neurons.

MeSH terms

Cellulose, Oxidized*
Hydrogels / chemistry
Neurons / metabolism
Spiral Ganglion* / metabolism

Substances

graphene oxide
Cellulose, Oxidized
Hydrogels