Magnetic carboxymethyl cellulose-silk fibroin hydrogel: A ternary nanobiocomposite exhibiting excellent biological activity and in vitro hyperthermia of cancer therapy

Adibeh Mohammadi; Reza Eivazzadeh-Keihan; Hooman Aghamirza Moghim Aliabadi; Amir Kashtiaray; Reza Ahangari Cohan; Milad Salimi Bani; Samira Komijani; Andisheh Etminan; Nabi Salehpour; Ali Maleki; Mohammad Mahdavi

doi:10.1016/j.jbiotec.2023.04.001

Magnetic carboxymethyl cellulose-silk fibroin hydrogel: A ternary nanobiocomposite exhibiting excellent biological activity and in vitro hyperthermia of cancer therapy

J Biotechnol. 2023 Apr 10:367:71-80. doi: 10.1016/j.jbiotec.2023.04.001. Epub 2023 Apr 5.

Affiliations

¹ Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, Iran.
² Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran. Electronic address: reza.tab_chemist@yahoo.com.
³ Advanced Chemical Studies Lab, Department of Chemistry, K. N. Toosi University of Technology, Tehran, Iran.
⁴ Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran.
⁵ Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan, Isfahan, Iran.
⁶ Biotechnology Research Center, Pasteur Institute of Iran, No. 358, 12 Farvardin St., 1316943551 Tehran, Iran; Department of Biotechnology, School of Biology, Alzahra University, Tehran, Iran.
⁷ School of Mechanical Engineering,Iran University of Science and Technology (IUST), Tehran, Iran.
⁸ Department of Medical Biotechnology, Pasteur Institute of Iran, Tehran, Iran.
⁹ Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, Iran. Electronic address: maleki@iust.ac.ir.
¹⁰ Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: momahdavi@sina.tums.ac.ir.

PMID: 37028560
DOI: 10.1016/j.jbiotec.2023.04.001

Abstract

In this work, a magnetic nanobiocomposite scaffold based on carboxymethylcellulose (CMC) hydrogel, silk fibroin (SF), and magnetite nanoparticles was fabricated. The structural properties of this new magnetic nanobiocomposite were characterized by various analyses such as FT-IR, XRD, EDX, FE-SEM, TGA and VSM. According to the particle size histogram, most of the particles were between 55 and 77 nm and the value of saturation magnetization of this nanobiocomposite was reported 41.65 emu.g^{- 1}. Hemolysis and MTT tests showed that the designed magnetic nanobiocomposite was compatible with the blood. In addition, the viability percentage of HEK293T normal cells did not change significantly, and the proliferation rate of BT549 cancer cells decreased in its vicinity. EC50 values for HEK293T normal cells after 48 h and 72 h were 3958 and 2566, respectively. Also, these values for BT549 cancer cells after 48 h and 72 h were 0.4545 and 0.9967, respectively. The efficiency of fabricated magnetic nanobiocomposite was appraised in a magnetic fluid hyperthermia manner. The specific absorption rate (SAR) of 69 W/g (for the 1 mg/mL sample at 200 kHz) was measured under the alternating magnetic field (AMF).

Keywords: Biological properties; Carboxymethyl Cellulose Hydrogel; Hyperthermia; Magnetic nanobiocomposite; Silk fibroin.

MeSH terms

Carboxymethylcellulose Sodium / chemistry
Carboxymethylcellulose Sodium / pharmacology
Fibroins* / chemistry
Fibroins* / pharmacology
HEK293 Cells
Humans
Hydrogels
Hyperthermia, Induced*
Magnetic Phenomena
Neoplasms* / drug therapy
Spectroscopy, Fourier Transform Infrared

Substances

Fibroins
Hydrogels
Carboxymethylcellulose Sodium