Evaluation of natural protein-based nanofiber composite photocrosslinking hydrogel for skin wound regeneration

Fan Yu; Jianfeng Pan; Atta Ur Rehman Khan; Binan Zhao; Zhengchao Yuan; Pengfei Cai; Xiao-Long Li; Mohamed El-Newehy; Hany El-Hamshary; Yosry Morsi; Binbin Sun; Ruijun Cong; Xiumei Mo

doi:10.1016/j.colsurfb.2023.113292

Evaluation of natural protein-based nanofiber composite photocrosslinking hydrogel for skin wound regeneration

Colloids Surf B Biointerfaces. 2023 Jun:226:113292. doi: 10.1016/j.colsurfb.2023.113292. Epub 2023 Apr 3.

Authors

Fan Yu¹, Jianfeng Pan², Atta Ur Rehman Khan³, Binan Zhao², Zhengchao Yuan¹, Pengfei Cai¹, Xiao-Long Li⁴, Mohamed El-Newehy⁵, Hany El-Hamshary⁵, Yosry Morsi⁶, Binbin Sun⁷, Ruijun Cong⁸, Xiumei Mo⁹

Affiliations

¹ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Songjiang, Shanghai 201600, PR China.
² Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, PR China.
³ School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Songjiang, Shanghai 200237, PR China.
⁴ Department of Orthopedics, Changhai Hospital, Naval Military Medical University, Shanghai 200433, China.
⁵ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
⁶ Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Boroondara, VIC 3122, Australia.
⁷ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Songjiang, Shanghai 201600, PR China. Electronic address: binbin.sun@dhu.edu.cn.
⁸ Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, PR China. Electronic address: crjg@163.com.
⁹ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Songjiang, Shanghai 201600, PR China. Electronic address: xmm@dhu.edu.cn.

PMID: 37028231
DOI: 10.1016/j.colsurfb.2023.113292

Abstract

Protein based photocrosslinking hydrogels with nanofiber dispersions were reported to be an effective wound dressing. In this study, two kinds of protein (gelatin and decellularized dermal matrix) were modified to obtain GelMA and ddECMMA, respectively. Poly(ε-caprolactone) nanofiber dispersions (PCLPBA) and thioglycolic acid-modified chitosan (TCS) were added into GelMA solution and ddECMMA solution, respectively. After photocrosslinking, four kinds of hydrogel (GelMA, GTP4, DP and DTP4) were fabricated. The hydrogels showed excellent physico-chemical property, biocompatibility and negligible cytotoxicity. When applied on the full-thickness cutaneous deficiency of SD rats, hydrogel treated groups exhibited an enhanced wound healing effect than Blank group. Besides, the histological staining of H&E and Masson's showed that hydrogels groups with PCLPBA and TCS (GTP4 and DTP4) improved wound healing. Furthermore, GTP4 group performed better healing effect than other groups, which had great potential in skin wound regeneration.

Keywords: Nanofiber; Photocrosslinking hydrogel; Protein; Wound healing.

MeSH terms

Animals
Gelatin / chemistry
Hydrogels* / chemistry
Nanofibers* / chemistry
Rats
Rats, Sprague-Dawley
Skin
Wound Healing

Substances

Hydrogels
Gelatin