Ultrasensitive visual detection of the food-borne pathogen via MOF encapsulated enzyme

Jianwei Liang; Zhiyang Huang; Kuiyu Wang; Liren Zhang; Yuhao Wan; Tao Yang; Hui Zeng

doi:10.1016/j.talanta.2023.124503

Ultrasensitive visual detection of the food-borne pathogen via MOF encapsulated enzyme

Talanta. 2023 Jul 1:259:124503. doi: 10.1016/j.talanta.2023.124503. Epub 2023 Apr 3.

Authors

Jianwei Liang¹, Zhiyang Huang², Kuiyu Wang², Liren Zhang², Yuhao Wan², Tao Yang³, Hui Zeng⁴

Affiliations

¹ School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai, 519082, China. Electronic address: liangjw58@mail2.sysu.edu.cn.
² School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai, 519082, China.
³ School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai, 519082, China. Electronic address: yangtao25@mail.sysu.edu.cn.
⁴ School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai, 519082, China; Guangdong Youkai Science and Technology Co., Ltd., Foshan, 528000, China. Electronic address: zenghui5@mail.sysu.edu.cn.

PMID: 37027932
DOI: 10.1016/j.talanta.2023.124503

Abstract

Various methods have been made to achieve sensitive detection (10 CFU/mL) of Escherichia coli O157:H7 (E. coli) in real samples, however, they are complex, time-consuming, or instrument-dependent. Enzyme-catalyzed reactions are one of the most efficient methods to amplify signals for sensitive detection. ZIF-8 owning stability, porosity, and high specific area are suitable for embedding enzymes which can effectively protect enzyme activity and thus improve detection sensitivity. Herein, a simple visual assay of E. coli with the limits of detection of 1 CFU/mL was developed based on this stable enzyme-catalyzed amplified system. A microbial safety test of milk, orange juice, seawater, cosmetic, and hydrolyzed yeast protein, was successfully performed with the limits of detection of 10 CFU/mL by the naked eye. And this bioassay possessed high selectivity and stability making the developed detection method practically promising.

Keywords: Instrument-free; Ultrasensitive; Visual detection; ZIF-8.

MeSH terms

Animals
Colony Count, Microbial
Escherichia coli O157*
Food Microbiology
Milk*