Heat-related biomarkers: Focus on the correlation of troponin I and 70 kDa heat shock protein

Emina Dervišević; Sabaheta Hasić; Muhamed Katica; Lejla Dervišević; Zurifa Ajanović; Adis Salihbegović

doi:10.1016/j.heliyon.2023.e14565

Heat-related biomarkers: Focus on the correlation of troponin I and 70 kDa heat shock protein

Heliyon. 2023 Mar 25;9(4):e14565. doi: 10.1016/j.heliyon.2023.e14565. eCollection 2023 Apr.

Authors

Emina Dervišević¹, Sabaheta Hasić², Muhamed Katica³, Lejla Dervišević⁴, Zurifa Ajanović⁴, Adis Salihbegović¹

Affiliations

¹ Department of Forensic Medicine, Faculty of Medicine, University of Sarajevo, 71 000 Sarajevo, Bosnia and Herzegovina.
² Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
³ Veterinary Clinical Pathology, Veterinary Faculty University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
⁴ Department of Human Anatomy, Faculty of Medicine, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.

Abstract

Heat-related biomarkers: Focus on the correlation of troponin I and 70 kDa heat shock protein Abstract Introduction: There is intensive research related to the forensic importance of biomarkers that would be the standard for postmortem damage to cardiomyocytes and the mechanism of the resulting damage. The aim of the research was to examinate the forensic-medical significance of serum levels of biomarkers as detectors of terminal hyperthermic damage to the myocardium.

Material and method: 40 laboratory animals were divided into groups: the first group was the control (n = 8) exposed to a physiological temperature of 37 °C, the second group was divided into two subgroups: antemortem (n = 8) and postmortem (n = 8), which included a exposure temperature of 41 °C and the third group was divided into two subgroups: antemortem (n = 8) and postmortem (n = 8), which included a exposure temperature of 44 °C. The concentration of cardiac TnI and Hsp70 was resoluted in serum by immunochemical enzyme-labeled immunoabsorption method.

Results: A positive correlation was found between the temperature measured at the time of death and the serum values of cTnI (p = 0.02), in G41, and Hsp70 values did not significantly correlate with the core temperature in this group, p > 0.005. A positive correlation was significant between the concentration of Hsp 70 and the body temperature of rats in the group of rats with a fatal outcome was determined, p = 0.03.

Conclusion: Changes in the concentration of cTnI and Hsp70 in rat serum may indicate hyperthermic damage to the myocardium in the Wistar rat model of heat stroke.

Keywords: Biomarkers; Experimental; Heat stroke; cTnI; hsp70.