A 384-Well Microtiter Plate Model for Candida Biofilm Formation and Its Application to High-Throughput Screening

Methods Mol Biol. 2023:2658:53-64. doi: 10.1007/978-1-0716-3155-3_5.

Abstract

Candidiasis, infections caused by Candida spp., represents one of the most common nosocomial infections afflicting an expanding number of compromised patients. Antifungal therapeutic options are few and show limited efficacy. Moreover, biofilm formation is frequently associated with different manifestations of candidiasis and further complicates therapy. Thus, there is an urgent need for new effective therapeutic agents, particularly those with anti-biofilm activity. Here we describe the development of a novel, simple, fast, economical, and highly reproducible 384-well microtiter plate model for the formation of both Candida albicans and Candida auris biofilms and its application in high-throughput screening (HTS) techniques.

Keywords: Antifungals; Biofilms; Candida; Candidiasis; High throughput screening.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology
  • Biofilms
  • Candida albicans
  • Candida*
  • Candidiasis* / drug therapy
  • Candidiasis* / microbiology
  • High-Throughput Screening Assays / methods
  • Humans
  • Microbial Sensitivity Tests

Substances

  • Antifungal Agents