Mineralogical characteristics of root iron plaque and its functional mechanism for regulating Cr phytoextraction of hyperaccumulator Leersia hexandra Swartz

Abstract

Leersia hexandra Swartz (L. hexandra) is a promising hyperaccumulator for Cr pollution remediation, but whether its Cr phytoextraction is subject to the root surface-attached iron plaque (IP) remains unclear. In this research, the natural and artificial IPs were proven to be comprised of small amounts of exchangeable Fe as well as carbonate Fe, and dominantly Fe minerals involving amorphous two-line ferrihydrite (Fh), poorly crystalline lepidocrocite (Le) and highly crystalline goethite (Go). The Fe content in the artificial IPs augmented with increasing induced Fe(II) concentration, and the 50 mg/L Fe(II) led to the identical Fe content and different component proportions of artificial IP (Fe50) and natural IP. Fh was consisted of highly aggregated nanoparticles, and the aging of Fh caused its phase conversion to rod-like Le and Go. The Cr(VI) adsorption results of Fe minerals corroborated the coordination of Cr(VI) onto the Fh surface and the significantly greater equilibrium Cr(VI) adsorption amount of Fh over Le and Go. The greatest Cr(VI) reduction capacity of Fh among three Fe minerals was found to be related to its most abundant surface-adsorbed Fe(II) content. The results of hydroponic experiment of L. hexandra showed that the presence of IP facilitated the Cr(VI) removal by L. hexandra during the cultivation period of 10-45 days, and consequently, compared to the Fe0 group (without IP), around 60% of increase in the Cr accumulation of shoots was achieved by Fe50 group. The findings of this work are conductive to furthering our understanding of IP-regulated Cr phytoextraction of L. hexandra.

Keywords: Cr(VI) removal; Fe minerals; Iron plaque (IP); Leersia hexandra Swartz (L. hexandra); Phytoextraction.