Vanadium(IV) and vanadium(V) complexation by succinic acid studied by affinity capillary electrophoresis. Simultaneous injection of two analytes in equilibrium studies

Abstract

The interaction of V(IV) and V(V) with succinic acid was investigated by affinity capillary electrophoresis (ACE) in aqueous acid solutions at pH values 1.5, 2.0 and 2.4, and different ligand concentrations. V(IV) and V(V) form protonated complexes with succinic acid ligand at this pH range. The logarithms of the stability constants, measured at 0.1 mol L^-1 (NaClO₄/HClO₄) ionic strength and 25 °C, are logβ₁₁₁=7.4 ± 0.2 and logβ₁₂₂=14.1 ± 0.5 for V(IV), and logβ₁₁₁=7.3 ± 0.1 for V(V), respectively. The stability constant values, extrapolated to zero ionic strength with the Davies equation, are logβ°₁₁₁=8.3 ± 0.2 and logβ°₁₂₂=15.6 ± 0.5 for V(IV) and logβ°₁₁₁=7.9 ± 0.1 for V(V). The application of ACE to the simultaneous equilibria of V(IV) and V(V) (injection of two analytes) was also attempted. When the results were compared with those obtained when introducing only one analyte in the capillary, using the traditional version of the method, similar stability constants and precision are obtained. The possibility of studying two analytes simultaneously decreases the time needed for the determination of the constants; this feature is especially valuable when working with hazardous materials or when only small quantities of ligand are available.

Keywords: Affinity capillary electrophoresis; Complexation; Stability constants; Succinic acid; Vanadium.