A new strategy has been developed to fabricate a bovine haemoglobin surface-imprinted core-shell nanocomposite adsorbent, demonstrating superparamagnetism via emulsion-free and sol-gel techniques. The obtained magnetic surface-imprinted polymers (MSIPs) possess a porous core-shell nanocomposite structure with a remarkable imprinted recognition ability for template protein in aqueous medium. The MSIPs display higher affinity, adsorption efficiency and selectivity, for template protein compared to the non-target protein. The morphology, adsorption, and recognition properties of the MSIPs have been evaluated by using several characterisation techniques, such as scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, and vibrating sample magnetometry. The results show the average diameter of MSIPs ranging between 400 and 600 nm with a saturation magnetization value of 52.6 emu/g and adsorption capacity of 43.75 mg/g. And because the obtained MSIPs possessed easily accessible recognition sites and performed fast kinetics for template immobilization, it could achieve equilibrium within 60 min. All this revealed the potential application of this approach as an alternative methodology for the generation of protein imprinted biomaterials.
Keywords: Bovine haemoglobin; Emulsion-free polymerization; Magnetic core-shell nanocomposites; Protein surface-imprinted polymer; Sol-gel technique.
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