Interaction and association analysis of malting related traits in barley

Irfan Iqbal; Zeratsion Abera Desta; Rajiv Kumar Tripathi; Aaron Beattie; Ana Badea; Jaswinder Singh

doi:10.1371/journal.pone.0283763

Interaction and association analysis of malting related traits in barley

PLoS One. 2023 Apr 4;18(4):e0283763. doi: 10.1371/journal.pone.0283763. eCollection 2023.

Authors

Irfan Iqbal¹, Zeratsion Abera Desta¹, Rajiv Kumar Tripathi¹, Aaron Beattie², Ana Badea³, Jaswinder Singh¹

Affiliations

¹ Plant Science Department, McGill University, Quebec, Canada.
² Crop Development Centre, University of Saskatchewan, Saskatoon, Canada.
³ Brandon Research and Development Centre, Agriculture and Agri-Food Canada, Brandon, Canada.

Abstract

Barley is considered as a foundation of the brewing and malting industry. Varieties with superior malt quality traits are required for efficient brewing and distillation processes. Among these, the Diastatic Power (DP), wort-Viscosity (VIS), β-glucan content (BG), Malt Extract (ME) and Alpha-Amylase (AA) are controlled by several genes linked to numerous quantitative trait loci (QTL), identified for barley malting quality. One of the well-known QTL, QTL2, associated with barley malting trait present on chromosome 4H harbours a key gene, called as HvTLP8 that has been identified for influencing the barley malting quality through its interaction with β-glucan in a redox-dependent manner. In this study, we examined to develop a functional molecular marker for HvTLP8 in the selection of superior malting cultivars. We first examined the expression of HvTLP8 and HvTLP17 containing carbohydrate binding domains in barley malt and feed varieties. The higher expression of HvTLP8 prompted us to further investigate its role as a marker for malting trait. By exploring the 1000 bp downstream 3' UTR region of HvTLP8, we found single nucleotide polymorphism (SNP) in between Steptoe (feed variety) and Morex (malt variety), which was further validated by Cleaved Amplified Polymorphic Sequence (CAPS) marker assay. Analysis of 91 individuals from the Steptoe x Morex doubled haploid (DH) mapping population revealed CAPS polymorphism in HvTLP8. Highly significant (p<0.001) correlations among ME, AA and DP malting traits were observed. The correlation coefficient (r) between these traits ranged from 0.53 to 0.65. However, the polymorphism in HvTLP8 did not correlate effectively with ME, AA, and DP. Altogether, these findings will help us to further design the experiment regarding the HvTLP8 variation and its association with other desirable traits.

Copyright: © 2023 Iqbal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping
Hordeum* / chemistry
Hordeum* / genetics
Humans
Phenotype
Polymorphism, Single Nucleotide
Quantitative Trait Loci / genetics

Grants and funding

Jaswinder Singh received the funding Brewing and Malting Barley Research Institute, Alberta Barley Commission, Saskatchewan Barley Development Commission and the Natural Sciences and Engineering Research Council of Canada for providing funding for this project Did the sponsors or funders play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript? NO.