Analysis of anthocyanins and total flavonoids content in functional rice and its recombination inbred lines

Xinyi Zhou; Wei Xie; Hao Jing; Junru Fu; Maomao Li; Jianmin Bian; Jie Xu; Yicong Cai; Haohua He; Dahu Zhou

doi:10.3389/fpls.2023.1113618

Analysis of anthocyanins and total flavonoids content in functional rice and its recombination inbred lines

Front Plant Sci. 2023 Mar 15:14:1113618. doi: 10.3389/fpls.2023.1113618. eCollection 2023.

Authors

Xinyi Zhou¹, Wei Xie¹, Hao Jing¹, Junru Fu¹, Maomao Li², Jianmin Bian¹, Jie Xu¹, Yicong Cai¹, Haohua He¹, Dahu Zhou¹

Affiliations

¹ Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of Agronomy, Jiangxi Agricultural University, Nanchang, China.
² National Engineering Research Center of Rice (Nanchang), Key Laboratory of Rice Physiology and Genetics of Jiangxi Province, Rice Research Institute, Jiangxi Academy of Agriculture Sciences, Nanchang, China.

Abstract

Anthocyanin is one of the flavonoids, which has strong antioxidant properties. Functional rice rich in anthocyanins can not only improve immunity, but also anti-radiation, beauty, anti-aging effect, very popular in the market. In this study, we used Zibaoxiangnuo 1 (ZBXN 1), a functional rice variety which is rich in total flavonoids and anthocyanins, as the experimental material to construct Recombination Inbred Lines (RILs) with Minghui63 (MH63), a variety without anthocyanins. The contents of anthocyanins and total flavonoids of RILs and two parents were determined for three consecutive generations. The average anthocyanin content of parent ZBXN 1 was 319.31 mg/kg, and the anthocyanin inheritance of RIL population was relatively stable, with 10 samples higher than ZBXN 1. In addition, there was no significant difference in the total flavonoids content between the two parents, the total flavonoids content of Z25 in RIL population was 0.33%. Based on these studies, we believe that ZBXN 1 has abundant and stable anthocyanins, which can be used as an intermediate breeding material for breeding high-quality varieties with high anthocyanins, and lay a foundation for breeding more anthocyanin-rich rice varieties.

Keywords: ZBXN 1; anthocyanins; flavonoids; functional rice; recombinant inbred lines (RILs).

Grants and funding

This work was supported by the National Natural Science Foundation of China (32101796), the Major Science and Technology Research and Development Project of Jiangxi Province (20203ABC28W013), and Science and Technology Project of Education Department of Jiangxi Province(GJJ2004409).