Reducing cadmium accumulation in shrimp using Escherichia coli with surface-displayed peptide

Abstract

Cadmium (Cd) is a hazardous metal that can accumulate in aquatic organisms and endanger human health via the food chain. In this study, genetic engineering was used to display a peptide with Cd-binding potential on the surface of Escherichia coli cells. This whole-cell adsorbent exhibited high affinity for Cd ions (Cd²⁺) in the solution. The Cd²⁺ adsorption capacity of the whole-cell adsorbent was three-fold that of the control cells in a 20 μM Cd²⁺ solution, and 97.2% ± 2.38% of the Cd²⁺ was removed. The whole-cell adsorbent was fed to shrimp (Neocaridina denticulata), and the surface-engineered E. coli successfully colonized the shrimp intestine, which showed significantly less Cd accumulation than the group not fed surface-engineered E. coli. The whole-cell adsorbent evidently protected shrimp from the toxicity of Cd²⁺ by adsorbing it. Moreover, the whole-cell adsorbent mitigated the changes in microbial community structure in the shrimp gut caused by the exposure of Cd²⁺. These findings suggest that this strategy is effective for controlling the contamination of Cd²⁺ in shrimp.

Keywords: Cadmium adsorption; Cell surface display; Gut microbiota; Shrimp.