A fluorescent probe based on phenothiazine for detection of ClO- with naked-eye color change properties

Chenglu Zhang; Yiming Wang; Xiangling Li; Shiru Nie; Chang Liu; Yang Zhang; Jinghao Guo

doi:10.1016/j.ab.2023.115131

A fluorescent probe based on phenothiazine for detection of ClO^- with naked-eye color change properties

Anal Biochem. 2023 Jun 1:670:115131. doi: 10.1016/j.ab.2023.115131. Epub 2023 Mar 29.

Authors

Chenglu Zhang¹, Yiming Wang², Xiangling Li², Shiru Nie², Chang Liu², Yang Zhang², Jinghao Guo²

Affiliations

¹ School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian, Liaoning, 116029, China. Electronic address: zhangchenglu@lnnu.edu.cn.
² School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian, Liaoning, 116029, China.

PMID: 37001597
DOI: 10.1016/j.ab.2023.115131

Abstract

Hypochlorite (ClO^-) plays a key role in life systems and it is necessary to develop an effective detection method. In view of the significant advantages of the fluorescent probe, we have synthesized a naked-eye recognition fluorescent probe NNCF for the detection of ClO^- based on phenothiazine and naphthalimide. The probe NNCF is sensitive (LOD = 9.5 nM) and fast for ClO^- (within 30 s), and its Stokes shift is as large as 161 nm. In addition, the probe NNCF has been successfully used for imaging detection of exogenous ClO^- in MCF-7 cells with low toxicity.

Keywords: Fluorescent probe; Hypochlorite; Naphthalimide; Phenothiazine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Eye Color
Fluorescent Dyes*
Humans
Hypochlorous Acid*
Phenothiazines

Substances

Fluorescent Dyes
Hypochlorous Acid
phenothiazine
Phenothiazines