Generation of a set of induced pluripotent stem cell lines from two Alzheimer disease patients carrying APOE4 (MLUi007-J; MLUi008-A) and healthy old donors carrying APOE3 (MLUi009-A; MLUi010-B) to study APOE in aging and disease

Stem Cell Res. 2023 Jun:69:103072. doi: 10.1016/j.scr.2023.103072. Epub 2023 Mar 15.

Abstract

Late-onset Alzheimer disease (LOAD) is the most frequent neurodegenerative disease, and the APOE ε4 allele is the most prominent risk factor for LOAD. Four human induced pluripotent stem cell (iPSC) lines MLUi007-J, MLUi008-B, MLUi009-A, and MLUi010-B were generated from LOAD patients and healthy matched donors by reprogramming of B-lymphoblastoid cells (B-LCLs) with episomal plasmids. The application of B-LCLs holds a great promise to model LOAD and other diseases because they can easily be generated from primary peripheral blood mononuclear cells (PBMCs) by infection with the Epstein-Barr virus (EBV).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging
  • Alzheimer Disease* / genetics
  • Alzheimer Disease* / metabolism
  • Apolipoprotein E3
  • Apolipoprotein E4 / genetics
  • Apolipoprotein E4 / metabolism
  • Epstein-Barr Virus Infections* / metabolism
  • Herpesvirus 4, Human
  • Humans
  • Induced Pluripotent Stem Cells* / metabolism
  • Leukocytes, Mononuclear
  • Neurodegenerative Diseases* / metabolism

Substances

  • Apolipoprotein E4
  • Apolipoprotein E3