Triblock copolymer-grafted restricted access materials with zwitterionic polymer outer layers for highly efficient extraction of fluoroquinolones and exclusion of proteins

Chunmiao Bo; Yinhai Li; Bin Liu; Bolin Gong; Xiaofan Tang; Guijuan Ma; Yan Li

doi:10.1016/j.foodchem.2023.135988

Triblock copolymer-grafted restricted access materials with zwitterionic polymer outer layers for highly efficient extraction of fluoroquinolones and exclusion of proteins

Food Chem. 2023 Aug 30:418:135988. doi: 10.1016/j.foodchem.2023.135988. Epub 2023 Mar 24.

Authors

Chunmiao Bo¹, Yinhai Li², Bin Liu², Bolin Gong², Xiaofan Tang², Guijuan Ma³, Yan Li²

Affiliations

¹ School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, China; Ningxia Key Laboratory of Solar Chemical Conversion Technology, North Minzu University, Yinchuan 750021,China; Key Laboratory for Chemical Engineering and Technology, State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, China. Electronic address: bocm-001@163.com.
² School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, China; Ningxia Key Laboratory of Solar Chemical Conversion Technology, North Minzu University, Yinchuan 750021,China; Key Laboratory for Chemical Engineering and Technology, State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, China.
³ NingXia Food Testing and Research Institute (Key Laboratory of Quality and Safety of Wolfberry and Wine for State Administration For Market Regulation), Yinchuan 750021, China.

PMID: 37001354
DOI: 10.1016/j.foodchem.2023.135988

Abstract

High-selectivity and high-exclusion restricted access materials (RAMs) benefit the analysis of biological samples. Herein, triblock copolymer-functionalized poly(4-vinylbenzyl chloride-co-divinylbenzene) (P_VBC/DVB) microspheres were prepared via the sequential surface-initiated atom radical polymerization of hydrophobic styrene (St), ionic vinylimidazole (VIm), and zwitterionic sulfobetaine methacrylate (SBMA), affording RAMs with multiple interaction-adsorption sites and zwitterionic polymer exclusion sites on the internal and external surfaces of P_VBC/DVB. The preferential extraction of fluoroquinolones (FQs) is realized based on the hydrophobic/π-π/ion exchange interactions due to the grafted poly-St-VIm, and the zwitterionic poly-SBMA block in the triblock copolymers can efficiently exclude various proteins. A sensitive detection method for FQs in chicken was established by solid phase extraction with RAMs as adsorbent combined with UPLC-MS/MS, achieving wide linearity (2.0-200.0 ng mL^-1), low limit of detection (0.5 μg kg^-1) and limit of quantification (1.5 μg kg^-1), and good inter- and intraday precision with satisfactory recoveries (104.1%-117.7% and 115.3%-121.2% with RSDs < 12%).

Keywords: Fluoroquinolones; Multiple-mode adsorption; Restricted access materials; Triblock copolymer; Zwitterionic polymer.

MeSH terms

Chromatography, Liquid
Fluoroquinolones* / analysis
Polymers* / chemistry
Solid Phase Extraction / methods
Tandem Mass Spectrometry

Substances

Fluoroquinolones
Polymers
sulfobetaine