In modern era, various inorganic fertilizers and pesticides are used as plant growth supplements in a variety of crop in order to gain maximum output and also reported as hazardous to mankind as well as environment. In addition, some of the plants died in initial phase of the growth after germination due to poor nutrient content of the soil or exposure to biotic stresses. In the beginning of sustainable agriculture, these chemical fertilizers were replaced with some alternative growth boosters such as organic fertilizers. In the present study, vermicompost was prepared using garden waste and cattle dung followed by analysis of various physico-chemical properties. Then tomato seeds were allowed to germinate in soil and supplemented with different doses of vermicompost (0-100%). The plants were harvested after 10 and 45 days of their germination and tissues were subjected to analysis of various morphological and biochemical parameters. Morphological parameters included root length, shoot length, root fresh weight, shoot fresh weight and number of leaves. Whereas biochemical parameters such as protein content, antioxidative enzymes (catalase, superoxide dismutase, ascorbate peroxidase, polyphenol oxidase), non-enzymatic antioxidants (ascorbic acid, glutathione, tocopherol), osmolytes (proline, carbohydrate), photosynthetic pigments (chlorophyll, carotenoid) and secondary metabolites (phenol, flavonoid, anthocyanin) were estimated on UV-visible spectrophotometer using standard protocols. Further, structural analysis of plant tissue was done using fourier transform infrared spectroscopy spectra (FTIR) and carbon hydrogen nitrogen (CHN) elemental analyzer. Results obtained from the present study revealed significant difference in all morphological and biochemical markers at both 10 and 45 days intervals of time. Further, growth of all plants was found to be directly proportional to the concentration of vermicompost and exposure duration. FTIR spectra and CHN analyses reveal the breakdown of various complex compounds and their transformation from Vcom amended soil to roots of plants. This is the first study in which significant changes were observed in growth, physiology and structural composition of tomato plants at two different exposure periods (10 and 45 days) under glass house conditions which further concluded that vermicompost has a significant potential for increasing plant growth.
Keywords: Antioxidative enzymes; CHN; FTIR; Phytoconstituents; Solanum lycopersicum; Vermicompost.
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