Dissemination of NDM-producing bacteria in Southern Brazil

Lavinia N V S Arend; Ricardo Bergamo; Fabiano B Rocha; Larissa Bail; Carmen Ito; Valter A Baura; Eduardo Balsanelli; Joël F Pothier; Fabio Rezzonico; Marcelo Pilonetto; Theo H M Smits; Felipe Francisco Tuon

doi:10.1016/j.diagmicrobio.2023.115930

Dissemination of NDM-producing bacteria in Southern Brazil

Diagn Microbiol Infect Dis. 2023 Jun;106(2):115930. doi: 10.1016/j.diagmicrobio.2023.115930. Epub 2023 Mar 6.

Authors

Affiliations

¹ Laboratory of Emerging Infectious Diseases, School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil; Public Health Central Laboratory-State of Paraná-LACEN/PR, Molecular Bacteriology Division, São José Dos Pinhais, Paraná, Brazil.
² State Health Department of Parana, Curitiba, Paraná, Brazil.
³ CGLAB General Coordination of Laboratories of Public Health, Ministry of Health, Brasilia, Federal District, Brazil.
⁴ Laboratory of Emerging Infectious Diseases, School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
⁵ Laboratory of Biochemistry, Polytechnic Center, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
⁶ Environmental Genomics and Systems Biology Research Group, Institute of Natural Resource Sciences (IUNR), Zurich University of Applied Sciences ZHAW, Wädenswil, Switzerland.
⁷ Public Health Central Laboratory-State of Paraná-LACEN/PR, Molecular Bacteriology Division, São José Dos Pinhais, Paraná, Brazil; Core for Advanced Molecular Investigation, Graduate Program in Health Sciences, School of Medicine, Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil.
⁸ Laboratory of Emerging Infectious Diseases, School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil. Electronic address: felipe.tuon@pucpr.br.

PMID: 37001228
DOI: 10.1016/j.diagmicrobio.2023.115930

Abstract

Background: The dissemination of NDM-1 carbapenemases (New Delhi Metallo-β-lactamase) is a global public health problem, mainly in developing countries. The aim of this study was to characterize the spread of NDM-producing bacteria in the Southern Brazilian states analyzing epidemiological, molecular, and antimicrobial susceptibility aspects.

Methods: A total of 10,684 carbapenem-resistant isolates of Enterobacterales, Pseudomonas spp. and Acinetobacter spp. obtained from several hospitals in eight cities in Southern Brazil were screened, and 486 NDM-producing bacteria were selected.

Results: The incidence varied from 0.5 to 77 cases/100.000 habitants. ST11, ST15, ST340 and ST674 were the most common in K. pneumoniae. A total of 5 plasmids were identified in one K. pneumoniae strain: Col440I, Col440II, IncFIA(HI1), IncFIB(K), IncFIB(pQil)/ IncFII(K), and IncR.

Conclusions: The number of patients with NDM-producing bacteria has increased in Southern Brazil, whose gene is present in different plasmids, explaining the expansion of this enzyme.

Keywords: Antimicrobial resistance; Carbapenemase; Enterobacterales; Klebsiella pneumoniae; Plasmids.

MeSH terms

Anti-Bacterial Agents* / pharmacology
Brazil / epidemiology
Carbapenems
Drug Resistance, Multiple, Bacterial / genetics
Humans
Klebsiella Infections* / microbiology
Klebsiella pneumoniae / genetics
Microbial Sensitivity Tests
Plasmids
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
beta-Lactamases
Carbapenems