Construction of a self-sufficient DNA circuit for amplified detection of kanamycin

Yumeng Li; Qingyang Si; Chuanyi Liu; Ziling Huang; Qingmin Chen; Tianhui Jiao; Xiaomei Chen; Quansheng Chen; Jie Wei

doi:10.1016/j.foodchem.2023.136048

Construction of a self-sufficient DNA circuit for amplified detection of kanamycin

Food Chem. 2023 Aug 30:418:136048. doi: 10.1016/j.foodchem.2023.136048. Epub 2023 Mar 27.

Authors

Yumeng Li¹, Qingyang Si¹, Chuanyi Liu¹, Ziling Huang¹, Qingmin Chen¹, Tianhui Jiao¹, Xiaomei Chen¹, Quansheng Chen², Jie Wei³

Affiliations

¹ College of Ocean Food and Biological Engineering, Jimei University, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China.
² College of Ocean Food and Biological Engineering, Jimei University, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China. Electronic address: chenqs@jmu.edu.cn.
³ College of Ocean Food and Biological Engineering, Jimei University, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China. Electronic address: jiewei@jmu.edu.cn.

PMID: 36996659
DOI: 10.1016/j.foodchem.2023.136048

Abstract

Improper use of kanamycin can lead to trace kanamycin residues in animal-derived foods, which can pose a potential threat to public health. Isothermal enzyme-free DNA circuits have provided a versatile toolbox for detecting kanamycin residues in complicated food samples, yet they are always limited by low amplification efficiency and intricate design. Herein, we present a simple-yet-robust nonenzymatic self-driven hybridization chain reaction (SHCR) amplifier for kanamycin determination with 5800-fold sensitivity over that of the conventional HCR circuit. The analyte kanamycin-activated SHCR circuitry can generate numerous new initiators to promote the reaction and improve the amplification efficiency, thus achieving an exponential signal gain. With precise target recognition and multilayer amplification capability, our self-sustainable SHCR aptasensor facilitated the highly sensitive and reliable analysis of kanamycin in buffer, milk, and honey samples, thus holding great potential for the amplified detection of trace contaminants in liquid food matrices.

Keywords: Aptasensor; Circuit; Exponential amplification; Kanamycin; Self-activation.

MeSH terms

Animals
Aptamers, Nucleotide* / chemistry
Biosensing Techniques*
DNA / chemistry
Kanamycin / analysis
Limit of Detection

Substances

Kanamycin
Aptamers, Nucleotide
DNA