CRISPR/Cas9-Mediated Genome Editing in Zebrafish

Jeffrey G Daniel; Xinge Yu; Allison C Ferguson; Jordan A Shavit

doi:10.1007/978-1-0716-2990-1_17

CRISPR/Cas9-Mediated Genome Editing in Zebrafish

Methods Mol Biol. 2023:2631:371-380. doi: 10.1007/978-1-0716-2990-1_17.

Authors

Jeffrey G Daniel¹, Xinge Yu¹, Allison C Ferguson¹, Jordan A Shavit^{2

3}

Affiliations

¹ Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA.
² Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA. jshavit@umich.edu.
³ Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA. jshavit@umich.edu.

PMID: 36995678
DOI: 10.1007/978-1-0716-2990-1_17

Abstract

The CRISPR/Cas9 system is a powerful tool for genome editing in zebrafish. This workflow takes advantage of the genetic tractability of zebrafish and will allow users to edit genomic sites and produce mutant lines using selective breeding. Established lines may then be employed by researchers for downstream genetic and phenotypic analyses.

Keywords: CRISPR/Cas9; Genome editing; Mutagenesis; Zebrafish.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
CRISPR-Cas Systems* / genetics
Gene Editing*
Genome
Zebrafish / genetics

Abstract

Publication types

MeSH terms

Grants and funding