Genotyping Genome-Edited Founders and Subsequent Generation

Abstract

Targeted nucleases allow the production of many types of genetic mutations directly in the early embryo. However, the outcome of their activity is a repair event of unpredictable nature, and the founder animals that are produced are generally of a mosaic nature. Here, we present the molecular assays and genotyping strategies that will support the screening of the first generation for potential founders and the validation of positive animals in the subsequent generation, according to the type of mutation generated.

Keywords: Allele QC; CRISPR-Cas9; Embryo/zygote engineering; Genetic engineering; Genome editing; Genotyping; Mouse models; Transgenesis.