Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.)

Truyen Quach; Hanh Nguyen; Olivia Meyer; Shirley J Sato; Tom Elmo Clemente; Ming Guo

doi:10.1007/978-1-0716-3131-7_17

Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.)

Methods Mol Biol. 2023:2653:273-285. doi: 10.1007/978-1-0716-3131-7_17.

Authors

Truyen Quach¹, Hanh Nguyen¹, Olivia Meyer², Shirley J Sato¹, Tom Elmo Clemente², Ming Guo³

Affiliations

¹ Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
² Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA.
³ Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA. mguo2@unl.edu.

PMID: 36995632
DOI: 10.1007/978-1-0716-3131-7_17

Abstract

Cas9-based genome editing is a powerful genetic tool for loci specifically targeted for genome modification. This chapter describes up-to-date protocols using Cas9-based genome editing technology, including vector construction with GoldenBraid assembly, Agrobacterium-mediated soybean transformation, and identification of editing in the genome.

Keywords: Cas9; Genome editing; GoldenBraid assembly; Soybean; Transformation.

MeSH terms

Alleles
CRISPR-Cas Systems / genetics
Gene Editing* / methods
Genotype
Glycine max* / genetics