The protocol presented here is to derive, maintain, and differentiate human pluripotent stem cells into skeletal muscle progenitor/stem cells (myogenic progenitors) using a sphere-based culture approach. This sphere-based culture is an attractive method for maintaining progenitor cells due to their longevity and the presence of cell-cell interactions and molecules. Large numbers of cells can be expanded in culture using this method, which represents a valuable source for cell-based tissue modeling and regenerative medicine.
Keywords: EZ spheres; Human embryonic stem cells; Human induced pluripotent stem cells; Human pluripotent stem cells; Myogenic progenitors; Skeletal muscle; Skeletal myotubes.
© 2023. Springer Science+Business Media, LLC, part of Springer Nature.