Extra Eyelid-Derived Muscle Stem Cells

Takahiko Sato; Yukito Yamanaka; Morio Ueno; Chie Sotozono

doi:10.1007/978-1-0716-3036-5_2

Extra Eyelid-Derived Muscle Stem Cells

Methods Mol Biol. 2023:2640:13-19. doi: 10.1007/978-1-0716-3036-5_2.

Authors

Takahiko Sato^{1

2

3}, Yukito Yamanaka³, Morio Ueno³, Chie Sotozono³

Affiliations

¹ International Center for Cell & Gene Therapy, Fujita Health University, Toyoake, Japan.
² Department of Anatomy, Fujita Health University, Toyoake, Japan.
³ Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.

PMID: 36995584
DOI: 10.1007/978-1-0716-3036-5_2

Abstract

Skeletal muscle stem cells (MuSCs) have been proposed as suitable candidates for cell therapy to muscular disorders since they exhibit a good capacity for myogenic regeneration. However, for better therapeutic outcomes, it is necessary to isolate human MuSCs from a suitable tissue source that possess high myogenic differentiation. In this context, isolated CD56+CD82+ cells from extra eyelid tissues were tested in vitro myogenic differentiation potential. Primary human myogenic cells derived from extra eyelids including orbicularis oculi, might be good candidates for human muscle stem cell-based research.

Keywords: Blepharoplasty; CD56; CD82; Muscle stem cells; Orbicularis ocular muscle; Pax7; Skeletal muscle.

MeSH terms

Cell Differentiation
Eyelids*
Humans
Muscle Fibers, Skeletal*
Muscle, Skeletal / physiology
Stem Cells