Test-retest reliability of high-resolution surface electromyographic activities of facial muscles during facial expressions in healthy adults: A prospective observational study

Vanessa Trentzsch; Nadiya Mueller; Martin Heinrich; Anna-Maria Kuttenreich; Orlando Guntinas-Lichius; Gerd Fabian Volk; Christoph Anders

doi:10.3389/fnhum.2023.1126336

Test-retest reliability of high-resolution surface electromyographic activities of facial muscles during facial expressions in healthy adults: A prospective observational study

Front Hum Neurosci. 2023 Mar 13:17:1126336. doi: 10.3389/fnhum.2023.1126336. eCollection 2023.

Authors

Vanessa Trentzsch^{1

2}, Nadiya Mueller^{1

2}, Martin Heinrich^{2

3

4}, Anna-Maria Kuttenreich^{2

3

4}, Orlando Guntinas-Lichius^{2

3

4}, Gerd Fabian Volk^{2

3

4}, Christoph Anders¹

Affiliations

¹ Division Motor Research, Pathophysiology and Biomechanics, Department of Trauma, Hand and Reconstructive Surgery, Jena University Hospital, Friedrich Schiller University Jena, Jena, Germany.
² Department of Otorhinolaryngology, Jena University Hospital, Friedrich Schiller University Jena, Jena, Germany.
³ Facial-Nerve-Center Jena, Jena University Hospital, Jena, Germany.
⁴ Center for Rare Diseases, Jena University Hospital, Jena, Germany.

Abstract

Objectives: Surface electromyography (sEMG) is a standard method for psycho-physiological research to evaluate emotional expressions or in a clinical setting to analyze facial muscle function. High-resolution sEMG shows the best results to discriminate between different facial expressions. Nevertheless, the test-retest reliability of high-resolution facial sEMG is not analyzed in detail yet, as good reliability is a necessary prerequisite for its repeated clinical application.

Methods: Thirty-six healthy adult participants (53% female, 18-67 years) were included. Electromyograms were recorded from both sides of the face using an arrangement of electrodes oriented by the underlying topography of the facial muscles (Fridlund scheme) and simultaneously by a geometric and symmetrical arrangement on the face (Kuramoto scheme). In one session, participants performed three trials of a standard set of different facial expression tasks. On one day, two sessions were performed. The two sessions were repeated two weeks later. Intraclass correlation coefficient (ICC) and coefficient of variation statistics were used to analyze the intra-session, intra-day, and between-day reliability.

Results: Fridlund scheme, mean ICCs per electrode position: Intra-session: excellent (0.935-0.994), intra-day: moderate to good (0.674-0.881), between-day: poor to moderate (0.095-0.730). Mean ICC's per facial expression: Intra-session: excellent (0.933-0.991), intra-day: good to moderate (0.674-0.903), between-day: poor to moderate (0.385-0.679). Kuramoto scheme, mean ICC's per electrode position: Intra-session: excellent (0.957-0.970), intra-day: good (0.751-0.908), between-day: moderate (0.643-0.742). Mean ICC's per facial expression: Intra-session: excellent (0.927-0.991), intra-day: good to excellent (0.762-0.973), between-day: poor to good (0.235-0.868). The intra-session reliability of both schemes were equal. Compared to the Fridlund scheme, the ICCs for intra-day and between-day reliability were always better for the Kuramoto scheme.

Conclusion: For repeated facial sEMG measurements of facial expressions, we recommend the Kuramoto scheme.

Keywords: electromyography; emotional expression; face; facial palsy; mimic muscles; reliability—reproducibility of results.

Grants and funding

VT and NM received a doctoral scholarship by the Interdisziplinäres Zentrum für Klinische Forschung (IZKF) of the Jena University Hospital. OG-L acknowledges support by the Deutsche Forschungsgemeinschaft (DFG), grant no. GU-463/12-1.