Comparative effect of conductive and dielectric materials on methanogenesis from highly concentrated volatile fatty acids

Svetlana Shekhurdina; Elena Zhuravleva; Andrey Kovalev; Egor Andreev; Emil Kryukov; Natalia Loiko; Alexandra Laikova; Nadezhda Popova; Dmitriy Kovalev; Vivekanand Vivekanand; Yuriy Litti

doi:10.1016/j.biortech.2023.128966

Comparative effect of conductive and dielectric materials on methanogenesis from highly concentrated volatile fatty acids

Bioresour Technol. 2023 Jun:377:128966. doi: 10.1016/j.biortech.2023.128966. Epub 2023 Mar 27.

Authors

Affiliations

¹ Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences, 60 let Oktjabrja pr-t, 7, Bld. 2, 117312 Moscow, Russia; Department of Biology, Lomonosov Moscow State University, Vorob'jovy gory, 119899 Moscow, Russia.
² Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences, 60 let Oktjabrja pr-t, 7, Bld. 2, 117312 Moscow, Russia.
³ Federal Scientific Agroengineering Center VIM, 1st Institutsky proezd, 5, 109428 Moscow, Russia.
⁴ M.V. Lomonosov Moscow State University, Chemistry Faculty, Leninskie gory 1, Build. 3, 119991 Moscow, Russia.
⁵ IM Sechenov First Moscow State Medical University, 8-2 Trubetskaya str. 119435 Moscow, Russia.
⁶ Frumkin Institute of Physical Chemistry and Electrochemistry RAS, 31, Bld.4, Leninsky prospect, 119071 Moscow, Russia.
⁷ Centre for Energy and Environment, Malaviya National Institute of Technology Jaipur, Jaipur 302017, Rajasthan, India.
⁸ Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences, 60 let Oktjabrja pr-t, 7, Bld. 2, 117312 Moscow, Russia. Electronic address: litty-yuriy@mail.ru.

PMID: 36990327
DOI: 10.1016/j.biortech.2023.128966

Abstract

Various conductive materials and their dielectric counterparts were used to get deeper insights into contribution of direct interspecies electron transfer (DIET) in improving methanogenesis from highly concentrated volatile fatty acids (12.5 g/L). Potential CH₄ yield, maximum CH₄ production rate and lag phase were significantly (up to 1.4, 3.9 and 2.0 times, respectively) improved with addition of stainless-steel mesh (SM) and carbon felt (CF) compared to both control and dielectric counterparts (p < 0.05). k_app increased by 82% for SM and 63% for CF compared to control (p < 0.05). Short thick pili-like structures up to 150 nm in width were formed only in CF and SM biofilms, however, were more abundant for SM. Ureibacillus and Limnochordia specific for SM biofilms, and Coprothermobacter and Ca. Caldatribacterium for CF biofilms, were considered electrogenic. Promotion of DIET by conductive materials is governed by many factors, including specificity of electrogenic groups to material surface.

Keywords: Anaerobic digestion; Biofilms; Direct interspecies electron transfer; Electroactive microorganisms; Pili-like structures.

MeSH terms

Anaerobiosis
Bioreactors
Carbon
Electric Conductivity
Electron Transport
Fatty Acids, Volatile*
Methane*
Stainless Steel

Substances

Methane
Fatty Acids, Volatile
Carbon
Stainless Steel