Super-resolution dynamic tracking of cellular lipid droplets employing with a photostable deep red fluorogenic probe

Jianan Dai; Zihan Wu; Di Li; Guishan Peng; Guannan Liu; Ri Zhou; Chenguang Wang; Xu Yan; Fangmeng Liu; Peng Sun; Ji Zhou; Geyu Lu

doi:10.1016/j.bios.2023.115243

Super-resolution dynamic tracking of cellular lipid droplets employing with a photostable deep red fluorogenic probe

Biosens Bioelectron. 2023 Jun 1:229:115243. doi: 10.1016/j.bios.2023.115243. Epub 2023 Mar 21.

Authors

Jianan Dai¹, Zihan Wu², Di Li³, Guishan Peng¹, Guannan Liu¹, Ri Zhou¹, Chenguang Wang⁴, Xu Yan¹, Fangmeng Liu¹, Peng Sun¹, Ji Zhou⁵, Geyu Lu⁶

Affiliations

¹ State Key Laboratory of Integrated Optoelectronics, Key Laboratory of Advanced Gas Sensors of Jilin Province, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China.
² Guangzhou Computational Super-resolution Biotech Co. Ltd, Guangzhou, 510700, China.
³ School of Materials Science and Engineering, Jilin University, Changchun, 130012, China.
⁴ State Key Laboratory of Integrated Optoelectronics, Key Laboratory of Advanced Gas Sensors of Jilin Province, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China. Electronic address: wangchenguang@jlu.edu.cn.
⁵ State Key Laboratory of Integrated Optoelectronics, Key Laboratory of Advanced Gas Sensors of Jilin Province, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China; State Kay Lab of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, China.
⁶ State Key Laboratory of Integrated Optoelectronics, Key Laboratory of Advanced Gas Sensors of Jilin Province, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China; International Center of Future Science, Jilin University, Changchun, 130012, China. Electronic address: lugy@jlu.edu.cn.

PMID: 36989580
DOI: 10.1016/j.bios.2023.115243

Abstract

Lipid droplets (LDs) are critical organelles involved in many physiological processes in eukaryotic cells. To visualize and study LDs, particular the small/nascent LDs, the emerging super-resolution fluorescence imaging techniques with nanoscale resolution would be much more powerful in comparison to the conventional confocal/wide-field imaging techniques. However, directly limited by the availability of advanced LDs probes, super-resolution fluorescence imaging of LDs is a practically challenging task. In this context, a superior LDs fluorescent probe named Lipi-Deep Red is newly developed for structured illumination microscopy (SIM) super-resolution imaging. This fluorescent probe features with the advantages of strong deep red/NIR emission, fluorogenic character, high LDs specificity, and outstanding photostability. These advantages enable the fluorescent probe to be finely applied in SIM super-resolution imaging, e.g. time-lapse imaging (up to 1000 frames) to monitor the LDs dynamics at nanoscale (159 nm), two-color time-lapse imaging to discover the nearby contact/interaction between LDs and mitochondria. Consequently, the fusion processes of LDs are impressively visualized at a high spatial and temporal resolution. Two kinds of contact models between LDs and mitochondria (dynamic contact and stable contact) newly proposed in the recent literatures are successfully revealed.

Keywords: Fluorescence imaging; Fluorescent probes; Lipid droplets; Live-cell imaging; Super-resolution imaging.

MeSH terms

Biosensing Techniques*
Fluorescent Dyes / metabolism
Lipid Droplets* / metabolism
Microscopy, Fluorescence / methods
Mitochondria

Substances

Fluorescent Dyes