[Mechanism of Der f 1/IGF-1 nanoparticle promoting the production of regulatory T cell]

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2023 Apr;37(4):272-277. doi: 10.13201/j.issn.2096-7993.2023.04.007.
[Article in Chinese]

Abstract
in English, Chinese

Objective:To prepare PLGA nanoparticles loaded with Der f 1/IGF-1（Der f 1/IGF-1 NPs） and investigate their role in promoting the formation of Treg cells. Methods:NPs coated with Der f 1/IGF-1 were prepared by double emulsion method and their physicochemical properties and cumulative release rate in vitro were analyzed. After pretreatment, BMDC was divided into Saline group, Blank NPs group, Der f 1/IGF-1 group and Der f 1/IGF-1 NPs group. Determination of the expression of IL-10 and TGF-β in BMDC by ELISA. The number of Treg cells was detected by flow cytometry. Results:The results showed that Der f 1/IGF-1 NPs were spherical structures, with good dispersion, particle size less than 200 nm, negative charge and stable slow-release effect of Zeta potential. After BMDC pretreatment, the expression levels of TGF-β and IL-10 in BMDC cells in the Der f 1/IGF-1 NPs group were significantly increased compared with the Blank NPs group, and the difference was statistically significant（P<0.001）. After co-culture with CD4+ T cells, the proportion of Treg cells produced in the Der f 1/IGF-1 NPs group was significantly increased, and the difference was statistically significant（P<0.001）. Conclusion:Der f 1/IGF-1 NPs can induce Treg cell generation in vitro. This study provides a new and more effective method for the reconstruction of immune tolerance dysfunction.

目的：旨在制备负载Der f 1/IGF-1的聚乳酸-乙醇酸共聚物（poly lactide-co-glycolide，PLGA）纳米颗粒（Der f 1/IGF-1 NPs），探讨其促进Treg细胞生成的作用。 方法：通过复乳法制备包裹Der f 1/IGF-1的NPs并分析其理化性质及体外累计释放率；预处理BMDC将细胞分为Saline组、Blank NPs组、Der f 1/IGF-1组以及Der f 1/IGF-1 NPs组；利用ELISA实验测定IL-10及TGF-β的表达；采用流式细胞术检测Treg细胞的数量变化。 结果：Der f 1/IGF-1 NPs为球形结构，具有良好的分散性，粒径均<200 nm，Zeta电位为负电荷且具有稳定的缓释效果；BMDC预处理后，相比较Blank NPs组，Der f 1/IGF-1 NPs组，BMDC细胞TGF-β、IL-10的表达水平明显增高，差异有统计学意义（P<0.001）；且与CD4⁺T细胞共培养后，Der f 1/IGF-1 NPs组Treg细胞生成的比例明显提高，差异有统计学意义（P<0.001）。 结论：Der f 1/IGF-1 NPs在体外实验中可诱导Treg细胞生成。本研究为重建免疫耐受功能失常提供了一种新的、更有效的方法。.

Keywords: DC cells; nanoparticles; regulatory T cell.

Publication types

  • English Abstract

MeSH terms

  • Drug Carriers / chemistry
  • Humans
  • Insulin-Like Growth Factor I
  • Interleukin-10 / metabolism
  • Nanoparticles* / chemistry
  • Particle Size
  • T-Lymphocytes, Regulatory* / metabolism
  • Transforming Growth Factor beta

Substances

  • Dermatophagoides farinae antigen f 1
  • Interleukin-10
  • Insulin-Like Growth Factor I
  • Transforming Growth Factor beta
  • Drug Carriers

Grants and funding

深圳市科技计划项目(No：JCYJ20220530154013030)