Solution of the Drug Resistance Problem of Escherichia coli with Silver Nanoparticles: Efflux Effect and Susceptibility to 31 Antibiotics

Ekaterina Nefedova; Nikolay N Shkil; Nikolay A Shkil; Diana Garibo; Roberto Luna Vazquez-Gomez; Alexey Pestryakov; Nina Bogdanchikova

doi:10.3390/nano13061088

Solution of the Drug Resistance Problem of Escherichia coli with Silver Nanoparticles: Efflux Effect and Susceptibility to 31 Antibiotics

Nanomaterials (Basel). 2023 Mar 17;13(6):1088. doi: 10.3390/nano13061088.

Authors

Ekaterina Nefedova^{1

2}, Nikolay N Shkil^{1

2}, Nikolay A Shkil¹, Diana Garibo^{3

4}, Roberto Luna Vazquez-Gomez⁵, Alexey Pestryakov², Nina Bogdanchikova⁴

Affiliations

¹ Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences, Novosibirsk 630501, Russia.
² Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk 634050, Russia.
³ Consejo Nacional de Ciencia y Tecnología, Ciudad de México 03940, Mexico.
⁴ Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada 22800, Baja California, Mexico.
⁵ Escuela de Ciencias de la Salud, Universidad Autónoma de Baja California, Ensenada 22890, Baja California, Mexico.

Abstract

The current work is a continuation of our studies focused on the application of nanoparticles of metallic silver (AgNPs) to address the global problem of antibiotic resistance. In vivo, fieldwork was carried out with 200 breeding cows with serous mastitis. Ex vivo analyses showed that after the cow was treated with an antibiotic-containing drug Dienomast^TM, E. coli sensibility to 31 antibiotics decreased by 27.3%, but after treatment with AgNPs, it increased by 21.2%. This could be explained by the 8.9% increase in the portion of isolates showing an efflux effect after Dienomast^TM treatment, while treatment with Argovit-C^TM resulted in a 16.0% drop. We verified the likeness of these results with our previous ones on S. aureus and Str. dysgalactiae isolates from mastitis cows processed with antibiotic-containing medicines and Argovit-C^TM AgNPs. The obtained results contribute to the recent struggle to restore the efficiency of antibiotics and to preserve the wide range of antibiotics on the world market.

Keywords: AgNPs; Escherichia coli; efflux effect; medicine resistance.

Grants and funding

This research was funded by CONACyT. México grant “Investigadoras e investigadores por México” No. 1073 and Russian Science Foundation and Tomsk Region, Grant Number 22-13-20032.