Trace Elements in Soy-Based Beverages: A Comprehensive Study of Total Content and In Vitro Bioaccessibility

Int J Environ Res Public Health. 2023 Mar 12;20(6):4986. doi: 10.3390/ijerph20064986.

Abstract

Soy-based beverages are one of the most consumed plant-based beverages, which have been used as a substitute for dairy products. Soy is a source of several nutrients (vitamins, minerals, and phenolic compounds, etc.) and its consumption is usually associated with several benefits, such as the prevention of cardiovascular diseases, cancer, and osteoporosis. However, non-essential trace elements can be found in these beverages. Thus, a comprehensive study concerning trace elements Al, As, Cd, Co, Cr, Cu, Fe, Li, Mn, Ni, Pb, Sb, Se, Sn, Sr, and Zn in soy-based beverages was proposed. In vitro digestion allowed to simulate the gastrointestinal juice (bioaccessibility) and the Caco-2 cells culture model was applied for the bioavailability assay. Trace elements measures were performed by inductively coupled plasma optical emission spectrometry (ICP OES). Multivariate analysis classified soy-based beverages according to their soy source (isolate protein, hydrosoluble extract, and beans); Al, Cu, Fe, Mn, Sr, Se, and Zn bioaccessible fractions corresponded to approximately 40%-80% of their total content, and soy-based beverages were found to be a good Fe, Se, and Zn source. However, our results showed risk exposure assessment from daily consumption of one glass of soy-based beverage can contribute to 3.5% and 0.9% of Al Provisional Tolerable Weekly Intake (PTWI) for children and adults, respectively.

Keywords: ICP OES; bioavailability; food safety; nutritional quality; soy-based beverages; trace elements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Beverages
  • Caco-2 Cells
  • Child
  • Humans
  • Minerals
  • Spectrum Analysis / methods
  • Trace Elements* / analysis

Substances

  • Trace Elements
  • Minerals

Grants and funding

This research was funded by the National Institute of Advanced Analytical Science and Technology (INCTAA) (CNPq 573894/2008-6 and FAPESP 2008/57808-1) and the National Council for Scientific and Technological Development (CNPq 407080/2021-0, 306054/2020-5 and S. Cadore productivity grant). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001.