Impact of Fulvic Acid and Acidithiobacillus ferrooxidan Inoculum Amount on the Formation of Secondary Iron Minerals

Int J Environ Res Public Health. 2023 Mar 8;20(6):4736. doi: 10.3390/ijerph20064736.

Abstract

The catalytic oxidation of Fe2+ by Acidithiobacillus ferrooxidan (A. ferrooxidans) and the synthesis of iron sulfate-based secondary minerals is considered to be of great significance to the treatment of acid mine drainage (AMD). Along these lines, in this work, the shaker experiment was carried out to study the underlying mechanism of the inoculation amount of fulvic acid (FA) and A. ferrooxidans on the synthesis process of secondary minerals. From the acquired results, it was demonstrated that the oxidation rate of Fe2+ increased with the increase in the concentration of fulvic acid in the range of 0.1-0.2 g/L. On top of that, the concentration of fulvic acid in the range of 0.3-0.5 g/L inhibited the activity of A. ferrooxidans. However, A. ferrooxidans retained its activity, and the complete oxidation time of Fe2+ was delayed. When the concentration of fulvic acid was 0.3 g/L, the TFe (total iron) precipitation efficiency was 30.2%. Interestingly, when 0.2 g/L fulvic acid was added to different inoculum systems, the incorporation of a higher inoculum amount of A. ferrooxidans led to an increased oxidation rate. On the contrary, the lower inoculum amount yielded a more obvious effect of the fulvic acid. From the mineralogical characteristics, it was also revealed that a fulvic acid concentration of 0.2 g/L and different inoculation amounts of A. ferrooxidans did not change the mineral facies, whereas pure schwertmannite was obtained.

Keywords: Acidithiobacillus ferrooxidan; acid mine drainage; fulvic acid; schwertmannite.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acidithiobacillus* / metabolism
  • Iron* / metabolism
  • Minerals / metabolism
  • Oxidation-Reduction

Substances

  • Iron
  • fulvic acid
  • Minerals

Supplementary concepts

  • Acidithiobacillus ferrooxidans

Grants and funding

This work was supported by Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology for Science and Education Combined with Science and Technology Innovation Base (Guilin), Guangxi ‘Bagui Scholar’ Construction Project, the Project of Guangxi Science and Technology Planning Project (Grant No. GuiKe-AD18126018), the Project of Foundation of Guilin University of Technology (GUTQDJJ 2005020).