Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

Jinghua Pan; Mengyi Zeng; Mengmeng Zhao; Liangzong Huang

doi:10.3389/fmicb.2023.1097905

Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

Front Microbiol. 2023 Mar 9:14:1097905. doi: 10.3389/fmicb.2023.1097905. eCollection 2023.

Authors

Jinghua Pan¹, Mengyi Zeng¹, Mengmeng Zhao^{1

2}, Liangzong Huang^{1

2}

Affiliations

¹ School of Life Science and Engineering, Foshan University, Foshan, China.
² Veterinary Teaching Hospital, Foshan University, Foshan, China.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes clinical syndromes typified as reproductive disorders in sows and respiratory diseases in piglets. PRRSV remains one of the most prevalent pathogens affecting the pig industry, because of its complex infection profile and highly heterogeneous genetic and recombination characteristics. Therefore, a rapid and effective PRRSV detection method is important for the prevention and control of PRRS. With extensive in-depth research on PRRSV detection methods, many detection methods have been improved and promoted. Laboratory methods include techniques based on virus isolation (VI), enzyme-linked immunosorbent assays (ELISA), indirect immunofluorescence assays (IFA), immunoperoxidase monolayer assays (IPMA), polymerase chain reaction (PCR), quantitative real-time PCR (qPCR), digital PCR (dPCR), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), clustered regularly interspaced short palindromic repeats (CRISPR), metagenomic next-generation sequencing (mNGS), and other methods. This study reviews the latest research on improving the main PRRSV detection methods and discusses their advantages and disadvantages.

Keywords: metagenomic next-generation sequencing; molecular biology; porcine reproductive and respiratory syndrome; serological assay; virus isolation.

Publication types

Review