Socs3b regulates the development and function of innate immune cells in zebrafish

Mohamed L Sobah; Aimee C Scott; Miranda Laird; Cassandra Koole; Clifford Liongue; Alister C Ward

doi:10.3389/fimmu.2023.1119727

Socs3b regulates the development and function of innate immune cells in zebrafish

Front Immunol. 2023 Mar 8:14:1119727. doi: 10.3389/fimmu.2023.1119727. eCollection 2023.

Authors

Mohamed L Sobah¹, Aimee C Scott², Miranda Laird¹, Cassandra Koole¹, Clifford Liongue^{1

2}, Alister C Ward^{1

2}

Affiliations

¹ School of Medicine, Deakin University, Geelong, VIC, Australia.
² Institue for Mental and Physical Health and Clinical Translation (IMPACT), Deakin University, Geelong, VIC, Australia.

Abstract

Introduction: Suppressor of cytokine signaling 3 (SOCS3) is a critical component of the negative feedback regulation that controls signaling by cytokines and other factors thereby ensuring that important processes such as hematopoiesis and inflammation occur at appropriate levels.

Methods: To gain further insights into SOCS3 function, the zebrafish socs3b gene was investigated through analysis of a knockout line generated using CRISPR/Cas9-mediated genome editing.

Results: Zebrafish socs3b knockout embryos displayed elevated numbers of neutrophils during primitive and definitive hematopoiesis but macrophage numbers were not altered. However, the absence of socs3b reduced neutrophil functionality but enhanced macrophage responses. Adult socs3b knockout zebrafish displayed reduced survival that correlated with an eye pathology involving extensive infiltration of neutrophils and macrophages along with immune cell dysregulation in other tissues.

Discussion: These findings identify a conserved role for Socs3b in the regulation of neutrophil production and macrophage activation.

Keywords: cytokine signaling; inflammation; innate immunity; socs3; zebrafish.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Immunity, Innate
Macrophages
Signal Transduction
Suppressor of Cytokine Signaling Proteins* / metabolism
Zebrafish*

Substances

Suppressor of Cytokine Signaling Proteins

Grants and funding

The authors recognize the support of PhD stipends (MS, ML) and project costs from Deakin University. The funder had no role in study design, data collection and interpretation or manuscript preparation.