A new strategy for improving cytotoxicity of a copper complex toward metastatic melanoma cells unveiled by EPR spectroscopy

Eduardo Guimarães Vieira; Rodrigo Boni Fazzi; Daniel O T A Martins; Alena M Sheveleva; Floriana Tuna; Ana Maria da Costa Ferreira

doi:10.1039/d2ra07266a

A new strategy for improving cytotoxicity of a copper complex toward metastatic melanoma cells unveiled by EPR spectroscopy

RSC Adv. 2023 Mar 24;13(14):9715-9719. doi: 10.1039/d2ra07266a. eCollection 2023 Mar 20.

Authors

Eduardo Guimarães Vieira^{1

2}, Rodrigo Boni Fazzi¹, Daniel O T A Martins², Alena M Sheveleva², Floriana Tuna², Ana Maria da Costa Ferreira¹

Affiliations

¹ Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo 05508-000 São Paulo-SP Brazil amdcferr@iq.usp.br.
² EPSRC National EPR Facility, Department of Chemistry and Photon Science Institute, University of Manchester Manchester M13 9PL UK floriana.tuna@manchester.ac.uk.

Abstract

A novel strategy of improving cytotoxicity against metastatic melanoma cells using an oxindolimine copper(ii) complex immobilized and dimerized on a modified Polyhedral Oligomeric Silsesquioxane (POSS) matrix was developed, as revealed by electron paramagnetic resonance (EPR) spectroscopy. An assured correlation between continuous-wave (CW) and pulsed EPR spectroscopies provided a complete characterization of the actual active species, its coordination environment, as well as the efficiency/selectivity of the bioconjugate materials.