Metagenomic characterization of biomethane transformation by lipid-catalyzed anaerobic fermentation of lignite

Zhenhong Chen; Bo Song; Hongyu Guo; Dapin Xia; Yidong Cai; Yongjun Wang; Weizhong Zhao

doi:10.1016/j.envres.2023.115777

Metagenomic characterization of biomethane transformation by lipid-catalyzed anaerobic fermentation of lignite

Environ Res. 2023 Jun 15:227:115777. doi: 10.1016/j.envres.2023.115777. Epub 2023 Mar 24.

Authors

Zhenhong Chen¹, Bo Song², Hongyu Guo³, Dapin Xia⁴, Yidong Cai⁵, Yongjun Wang⁶, Weizhong Zhao⁷

Affiliations

¹ Research Institute of Petroleum Exploration & Development, Beijing, 100083, China. Electronic address: chenzhenhong@petrochina.com.cn.
² School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo, 454000, China. Electronic address: 2604921166@qq.com.
³ School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo, 454000, China; Collaborative Innovation Center of Coal Work Safety and Clean High Efficiency Utilization, Henan Polytechnic University, Jiaozuo, 454000, China. Electronic address: ghy1026@126.com.
⁴ Mining Research Institute of Henan Polytechnic University, Jiaozuo, 454000, China. Electronic address: xiadp22@hpu.edu.cn.
⁵ School of Energy Resources, China University of Geosciences, Beijing, 100083, China. Electronic address: yidong.cai@cugb.edu.cn.
⁶ College of Computer Science and Technology, Henan Polytechnic University, Jiaozuo, 454000, China. Electronic address: hpuwyj@hpu.edu.cn.
⁷ Department of Environmental Engineering, Technical University of Denmark, DK-2800, Lyngby, Denmark. Electronic address: 279918580@qq.com.

PMID: 36966989
DOI: 10.1016/j.envres.2023.115777

Abstract

The present study aims at using lipid in a novel way to improve the efficiency of methane production from lignite anaerobic digestion. The obtained results showed an increase by 3.13 times of the cumulative biomethane content of lignite anaerobic fermentation, when 1.8 g lipid was added. The gene expression of functional metabolic enzymes was also found to be enhanced during the anaerobic fermentation. Moreover, the enzymes related to fatty acid degradation such as long-chain Acyl-CoA synthetase and Acyl-CoA dehydrogenase were increased by 1.72 and 10.48 times, respectively, which consequently, accelerated the conversion of fatty acid. Furthermore, the addition of lipid enhanced the carbon dioxide trophic and acetic acid trophic metabolic pathways. Hence, the addition of lipids was argued to promote the production of methane from lignite anaerobic fermentation, which provided a new insight for the conversion and utilization of lipid waste.

Keywords: Anaerobic fermentation; Biomethane; Lignite; Lipid; Metabolic pathways; Metagenome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anaerobiosis
Bioreactors
Catalysis
Fatty Acids* / metabolism
Fermentation
Methane*

Substances

Fatty Acids
Methane