Evaluation of cold atmospheric plasma for the decontamination of flexible endoscopes

R C Hervé; M G Kong; S Bhatt; H-L Chen; E E Comoy; J-P Deslys; T J Secker; C W Keevil

doi:10.1016/j.jhin.2023.03.013

Evaluation of cold atmospheric plasma for the decontamination of flexible endoscopes

J Hosp Infect. 2023 Jun:136:100-109. doi: 10.1016/j.jhin.2023.03.013. Epub 2023 Mar 23.

Authors

R C Hervé¹, M G Kong², S Bhatt², H-L Chen², E E Comoy³, J-P Deslys³, T J Secker⁴, C W Keevil⁴

Affiliations

¹ Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Southampton, UK. Electronic address: R.Herve@soton.ac.uk.
² Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA.
³ Commissariat à l'Energie Atomique et aux Energies Alternatives, Direction de la Recherche Fondamentale, Institut François Jacob, SEPIA, Université Paris-Saclay Fontenay-aux-Roses, France.
⁴ Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Southampton, UK.

PMID: 36965823
DOI: 10.1016/j.jhin.2023.03.013

Abstract

Background: Despite adherence to standard protocols, residues including live micro-organisms may remain on the various surfaces of reprocessed flexible endoscopes. Prions are infectious proteins that are notoriously difficult to eliminate.

Aim: To test the potential of cold atmospheric plasma (CAP) for the decontamination of various surfaces of flexible endoscopes, measuring total proteins and prion residual infectivity as indicators of efficacy.

Methods: New PTFE endoscope channels and metal test surfaces spiked with test soil or prion-infected tissues were treated using different CAP-generating prototypes. Surfaces were examined for the presence of residues using very sensitive fluorescence epimicroscopy. Prion residual infectivity was determined using the wire implant animal model and a more sensitive cell infectivity assay.

Findings: A CAP jet applied perpendicularly at close range on flat test surfaces removed soil within 3 min, but left microscopic residues and failed to eliminate prion infectivity according to the wire implant animal assay. The longitudinal gas flow from CAP prototypes developed for the treatment of long channels led to the displacement and sedimentation of residual soil towards the distal end, when applied alone. Observations of the plasma inside glass tubes showed temporal and spatial heterogeneity within a limited range. After the standard enzymatic manual pre-wash, 'CAP-activated' gas effluents prevented prion transmission from treated endoscope channels according to the prion infectivity cell assay.

Conclusion: CAP shows promising results as a final step for decontamination of surgical surfaces. Optimizing CAP delivery could further enhance CAP efficacy, offering a safe, chemical-free alternative for the reprocessing of all luminal flexible endoscope surfaces.

Keywords: Antimicrobial resistance; Biofilm; Cold atmospheric plasma; Decontamination; Luminal endoscopes; Proteins; Variant Creutzfeldt–Jakob disease.

MeSH terms

Animals
Decontamination* / methods
Endoscopes
Prions*

Substances

Prions