The authors studied the effects of gamma radiation and submaximal physical exercise on aldolase activity in healthy men erythrocytes. Twelve men aged 20-22 were examined. They underwent physical exercise at doses of 2 W/kg body weight for 15 minutes. Erythrocytes were exposed to gamma radiation (500 Gy doses) from 60Co source. The activity of aldolase in erythrocytes was estimated by Fermognost test. The submaximal physical exercise was indicated to increase the aldolase activity. Gamma radiation at 500 Gy dose was found to increase aldolase enzymatic activity in erythrocytes both a rest and after submaximal physical exercise.