Heterologous production of polycyclopropanated fatty acids and their methyl esters in Streptomyces

Kevin Yin; Pablo Cruz-Morales; Christopher M Whitford; Jay D Keasling

doi:10.1016/j.xpro.2023.102190

Heterologous production of polycyclopropanated fatty acids and their methyl esters in Streptomyces

STAR Protoc. 2023 Mar 22;4(2):102190. doi: 10.1016/j.xpro.2023.102190. Online ahead of print.

Authors

Kevin Yin¹, Pablo Cruz-Morales², Christopher M Whitford³, Jay D Keasling⁴

Affiliations

¹ Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, USA; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: kevin_yin@berkeley.edu.
² Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, USA; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; QB3 Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
³ The Novo Nordisk Foundation Center for Biosustainability, Technical University Denmark, Kemitorvet, Building 220, Kongens Lyngby 2800, Denmark.
⁴ Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, USA; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; QB3 Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemical and Biomolecular Engineering and Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; Center for Synthetic Biochemistry, Shenzhen Institutes for Advanced Technologies, Shenzhen 518055, P.R. China; The Novo Nordisk Foundation Center for Biosustainability, Technical University Denmark, Kemitorvet, Building 220, Kongens Lyngby 2800, Denmark. Electronic address: keasling@berkeley.edu.

Abstract

Polycyclopropanated (POP) compounds show promise as fuels as their energy density can be greater than jet and rocket fuels in current use, but realizing their full potential requires significant development. This protocol guides the production of polycyclopropanated fatty acids in Streptomyces; POP production in another host remains to be demonstrated. This method can serve as a baseline for further development of POP as well as other polyketide products. For complete details on the use and execution of this protocol, please refer to Cruz-Morales et al. (2022).¹.

Keywords: Biotechnology and Bioengineering; Chemistry; Energy; Mass Spectrometry; Microbiology; Molecular Biology.

Published by Elsevier Inc.