Live-Cell Imaging of Peroxisomal Calcium Levels and Dynamics

Yelena Sargsyan; Sven Thoms

doi:10.1007/978-1-0716-3048-8_14

Live-Cell Imaging of Peroxisomal Calcium Levels and Dynamics

Methods Mol Biol. 2023:2643:199-206. doi: 10.1007/978-1-0716-3048-8_14.

Authors

Yelena Sargsyan¹, Sven Thoms^{2

3

4}

Affiliations

¹ Department of Child and Adolescent Health, University Medical Center, Göttingen, Germany.
² Department of Child and Adolescent Health, University Medical Center, Göttingen, Germany. sven.thoms@uni-bielefeld.de.
³ Department for Biochemistry and Molecular Medicine, Medical School EWL, Bielefeld University, Bielefeld, Germany. sven.thoms@uni-bielefeld.de.
⁴ German Center of Cardiovascular Research (DZHK), Partner site Göttingen, Göttingen, Germany. sven.thoms@uni-bielefeld.de.

PMID: 36952187
DOI: 10.1007/978-1-0716-3048-8_14

Abstract

Calcium (Ca²⁺) is an intracellular messenger that plays an essential role in a variety of cellular processes ranging from early embryonic events to muscle contraction and neuron excitability. Measurement of cytosolic, endoplasmic reticulum (ER), and mitochondrial Ca²⁺ has contributed immensely to our understanding of cellular physiology. Here we describe the measurement of peroxisomal Ca²⁺ using ratiometric Ca²⁺ sensors, enabling measurement of absolute Ca²⁺ concentration and its dynamics in living cells.

Keywords: Calcium; FRET; Live-cell imaging; Peroxisome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calcium Channels / metabolism
Calcium Signaling / physiology
Calcium* / metabolism
Cytosol / metabolism
Endoplasmic Reticulum* / metabolism

Substances

Calcium
Calcium Channels