Introgression and targeting of the Pl37 and Pl38 genes for downy mildew resistance from wild Helianthus annuus and H. praecox into cultivated sunflower (Helianthus annuus L.)

Abstract

Two new downy mildew resistance genes, Pl₃₇ and Pl₃₈, were introgressed from wild sunflower species into cultivated sunflower and mapped to sunflower chromosomes 4 and 2, respectively Downy mildew (DM), caused by the oomycete pathogen Plasmopara halstedii (Farl.) Berl. & de Toni, is known as the most prevalent disease occurring in global sunflower production areas, especially in North America and Europe. In this study, we report the introgression and molecular mapping of two new DM resistance genes from wild sunflower species, Helianthus annuus and H. praecox, into cultivated sunflower. Two mapping populations were developed from the crosses of HA 89/H. annuus PI 435417 (Pop1) and CMS HA 89/H. praecox PRA-417 (Pop2). The phenotypic evaluation of DM resistance/susceptibility was conducted in the BC₁F₂-derived BC₁F₃ populations using P. halstedii race 734. The BC₁F₂ segregating Pop1 was genotyped using an Optimal GBS AgriSeq™ Panel consisting of 768 mapped SNP markers, while the BC₁F₂ segregating Pop2 was genotyped using a genotyping-by-sequencing approach. Linkage analysis and subsequent saturation mapping placed the DM resistance gene, designated Pl₃₇, derived from H. annuus PI 435417 in a 1.6 cM genetic interval on sunflower chromosome 4. Pl₃₇ co-segregated with SNP markers SPB0003 and C4_5738736. Similarly, linkage analysis and subsequent saturation mapping placed the DM resistance gene, designated Pl₃₈, derived from H. praecox PRA-417 in a 0.8 cM genetic interval on sunflower chromosome 2. Pl₃₈ co-segregated with seven SNP markers. Multi-pathotype tests revealed that lines with Pl₃₇ or Pl₃₈ are immune to the most prevalent and virulent P. halstedii races tested. Two germplasm lines, HA-DM15 with Pl₃₇ and HA-DM16 with Pl₃₈, were developed for use in sunflower DM-resistance breeding.