Self-assembly Induced Enhanced Electrochemiluminescence of Copper Nanoclusters Using DNA Nanoribbon Templates

Xiangyuan Ouyang; Yongli Wu; Linjie Guo; Le Li; Mo Zhou; Xinyi Li; Ting Liu; Yawen Ding; Huaiyu Bu; Gang Xie; Jianlei Shen; Chunhai Fan; Lihua Wang

doi:10.1002/anie.202300893

Self-assembly Induced Enhanced Electrochemiluminescence of Copper Nanoclusters Using DNA Nanoribbon Templates

Angew Chem Int Ed Engl. 2023 May 15;62(21):e202300893. doi: 10.1002/anie.202300893. Epub 2023 Apr 17.

Authors

Xiangyuan Ouyang¹, Yongli Wu¹, Linjie Guo^{2

3}, Le Li¹, Mo Zhou^{2

4}, Xinyi Li¹, Ting Liu¹, Yawen Ding¹, Huaiyu Bu¹, Gang Xie¹, Jianlei Shen⁵, Chunhai Fan⁵, Lihua Wang^{2

3

4}

Affiliations

¹ Xi'an Key Laboratory of Functional Supramolecular Structure and Materials, Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi, 710127, China.
² The Interdisciplinary Research Center, Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China.
³ Institute of Materials Biology, Department of Chemistry, College of Science, Shanghai University, Shanghai, 200444, China.
⁴ Zhangjiang Laboratory, Shanghai, 201210, China.
⁵ School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China.

PMID: 36951433
DOI: 10.1002/anie.202300893

Abstract

Copper nanoclusters (CuNCs) are attractive electrochemiluminescence (ECL) emitters as Cu is comparatively inexpensive, nontoxic, and highly abundant. However, their ECL yield is relatively low. Herein, we report that orderly self-assembly of CuNCs using DNA nanoribbon as the template (DNR/CuNCs) conferred the CuNCs with improved ECL properties compared with individual CuNCs in both annihilation and co-reactant processes. The DNR/CuNCs resulted in a high ECL yield of 46.8 % in K₂ S₂ O₈ , which was ≈68 times higher than that of individual CuNCs. This strategy was successfully extended to other ECL emitters, such as gold nanoclusters and the Ru(bpy)₃ ²⁺ /TPrA system. Furthermore, as an application of DNR/CuNCs, a DNR/CuNC-based ECL biosensor with higher sensitivity was constructed for dopamine determination (two orders of magnitude lower than that previously reported), showing that DNR/CuNCs have a potential for application in ECL bioanalysis as a new type of superior luminophore candidate.

Keywords: Biosensor; Copper Nanocluster; DNA Nanostructure; Electrochemiluminescence; Self-Assembly.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques* / methods
Copper
DNA / analysis
Electrochemical Techniques / methods
Luminescent Measurements / methods
Nanotubes, Carbon*

Substances

Copper
Nanotubes, Carbon
DNA