The modulation of light quality on carotenoid and tocochromanol biosynthesis in mung bean (Vigna radiata) sprouts

Yaoyao Cheng; Nan Xiang; Honglin Chen; Yihan Zhao; Lixia Wang; Xuzhen Cheng; Xinbo Guo

doi:10.1016/j.fochms.2023.100170

The modulation of light quality on carotenoid and tocochromanol biosynthesis in mung bean (Vigna radiata) sprouts

Food Chem (Oxf). 2023 Mar 7:6:100170. doi: 10.1016/j.fochms.2023.100170. eCollection 2023 Jul 30.

Authors

Yaoyao Cheng¹, Nan Xiang¹, Honglin Chen², Yihan Zhao¹, Lixia Wang², Xuzhen Cheng², Xinbo Guo¹

Affiliations

¹ School of Food Science and Engineering, Ministry of Education Engineering Research Centre of Starch & Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China.
² Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Abstract

This study aimed to identify the regulatory mechanisms of white, blue, red lights on carotenoid and tocochromanol biosynthesis in mung bean sprouts. Results showed that three lights stimulated the increase of the predominated lutein (3.2-8.1 folds) and violaxanthin (2.1-6.1 folds) in sprouts as compared with dark control, as well as β-carotene (20-36 folds), with the best yield observed under white light. Light signals also promoted α- and γ-tocopherol accumulation (up to 1.8 folds) as compared with dark control. The CRTISO, LUT5 and DXS (1.24-6.34 folds) exhibited high expression levels under light quality conditions, resulting in an overaccumulation of carotenoids. The MPBQ-MT, TC and TMT were decisive genes in tocochromanol biosynthesis, and were expressed up to 4.19 folds as compared with control. Overall, the results could provide novel insights into light-mediated regulation and fortification of carotenoids and tocopherols, as well as guide future agricultural cultivation of mung bean sprouts.

Keywords: BL, blue light; Biofortification; Biosynthesis; CK, dark control; Carotenoids; HPLC, high performance liquid chromatography; LEDs, light-emitting diodes; LHCII, light-harvesting complex of PSII; LQ, light quality; Light quality; MEP, methylerythritol phosphate; Mung bean; NASH, nonalcoholic steatohepatitis; PS, photosynthesis; PSII, photosystem II; PSs, photosystems; RL, red light; Tocopherols; VAD, vitamin A deficiency; WL, white light.