Rapid and ultrasensitive detection of acute kidney injury biomarkers CH3L1 and L-FABP using surface-enhanced Raman spectroscopy

Luyao Wang; Pei Ma; Hui Chen; Min Chang; Ping Lu; Nan Chen; Xuedian Zhang; Yanhua Li; Mingxing Sui

doi:10.1016/j.saa.2023.122604

Rapid and ultrasensitive detection of acute kidney injury biomarkers CH3L1 and L-FABP using surface-enhanced Raman spectroscopy

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jul 5:295:122604. doi: 10.1016/j.saa.2023.122604. Epub 2023 Mar 9.

Authors

Luyao Wang¹, Pei Ma², Hui Chen¹, Min Chang¹, Ping Lu¹, Nan Chen³, Xuedian Zhang⁴, Yanhua Li⁵, Mingxing Sui⁶

Affiliations

¹ Key Laboratory of Optical Technology and Instrument for Medicine, Ministry of Education, College of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
² Key Laboratory of Optical Technology and Instrument for Medicine, Ministry of Education, College of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China. Electronic address: peima@usst.edu.cn.
³ School of Electrical Engineering, Nantong University, Nantong 226019, China.
⁴ Key Laboratory of Optical Technology and Instrument for Medicine, Ministry of Education, College of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; Shanghai Institute of Intelligent Science and Technology, Tongji University, Shanghai 200092, China.
⁵ Department of Organ Transplantation, Shanghai Changhai Hospital, Shanghai 200433, China.
⁶ Department of Organ Transplantation, Shanghai Changhai Hospital, Shanghai 200433, China. Electronic address: mingxingsui@smmu.edu.cn.

PMID: 36947940
DOI: 10.1016/j.saa.2023.122604

Abstract

Chitinase 3-like 1 (CH3L1) and liver fatty acid binding protein (L-FABP) are promising biomarkers for the early diagnosis of acute kidney injury (AKI). Here, a highly sensitive method for the quantitative detection of CH3L1 and L-FABP by surface-enhanced Raman spectroscopy (SERS) based on graphene oxide/gold and silver core-shell nanoparticles (GO/Au@Ag NPs) was proposed. The results showed that such GO/Au@Ag substrate can achieve rapid sensing of CH3L1 and L-FABP with a wide response range (2 × 10^-1 to 2 × 10^-8 mg/mL and 1.2 × 10^-1 to 1.2 × 10^-8 mg/mL, respectively) and high sensitivity. The detection limits of CH3L1 and L-FABP were 1.21 × 10^-8 mg/mL and 0.62 × 10^-8 mg/mL, respectively. In addition, the simultaneous detection of the two biomarkers in serum was demonstrated, showing the feasibility of this method in the complex biological environment. The detection of CH3L1 and L-FABP will greatly improve the early diagnosis and intervention of AKI.

Keywords: Acute Kidney Injury; CH3L1; GO/Au@Ag NPs; L-FABP; SERS.

MeSH terms

Acute Kidney Injury* / diagnosis
Biomarkers
Chitinase-3-Like Protein 1 / analysis
Fatty Acid-Binding Proteins
Gold / chemistry
Humans
Metal Nanoparticles* / chemistry
Silver / chemistry
Spectrum Analysis, Raman / methods

Substances

Biomarkers
Fatty Acid-Binding Proteins
Gold
Silver
Chitinase-3-Like Protein 1