Influence of nitro ruthenium isomerization on photochemically induced nitric oxide release: Vasorelaxant activities

Alexia Marques Silva; Laísa Bonafim Negri; Juliana Cristina Biazzotto; Sergio de Paula Machado; Jeimison Duarte Santos; Jorge Fernandes Nasser Batista; Pedro Ivo S Maia; Victor Marcelo Deflon; Lusiane M Bendhack; Michael R Hamblin; Roberto S da Silva

doi:10.1016/j.jinorgbio.2023.112166

Influence of nitro ruthenium isomerization on photochemically induced nitric oxide release: Vasorelaxant activities

J Inorg Biochem. 2023 Jun:243:112166. doi: 10.1016/j.jinorgbio.2023.112166. Epub 2023 Mar 3.

Affiliations

¹ Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, United States. Electronic address: silva.242@osu.edu.
² Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Dermatology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: lnegri@mgh.harvard.edu.
³ Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: jmoraes@fcfrp.usp.br.
⁴ Instituto de Química, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, RJ, Brazil. Electronic address: sergiopm@iq.ufrj.br.
⁵ Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: jeimisonds@gmail.com.
⁶ Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: jorgenasser07@gmail.com.
⁷ Departamento de Química, Instituto de Ciências Exatas, Naturais e Educação, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil. Electronic address: pedro.maia@uftm.edu.br.
⁸ Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil. Electronic address: deflon@iqsc.usp.br.
⁹ Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: bendhack@usp.br.
¹⁰ Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein 2028, South Africa. Electronic address: hamblin.lab@gmail.com.
¹¹ Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Dermatology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: silva@usp.br.

PMID: 36947899
DOI: 10.1016/j.jinorgbio.2023.112166

Abstract

We have synthesized cis-[Ru(bpy)₂(NO₂-κN)L^n-]^(n-1) and cis-[Ru(bpy)₂(NO₂-κO)L ^n-]^(n-1) (bpy = 2,2'-bipyridine; k = indication of the coordinated center to Ruthenium; L = pyridine type ligand) by reacting cis-[Ru(bpy)₂(H₂O)L^n-]^(n-2) with sodium nitrite or conducting basic cis-[Ru(bpy)₂NO(L^n-)]^(n-3) hydrolysis. Photolysis at the metal-ligand charge transfer band (MLCT) of the isomers yielded nitric oxide (NO) as determined by NO measurement. The NO photorelease rates obtained upon 447 nm laser irradiation of the ruthenium complexes showed that cis-[Ru(bpy)₂(NO₂-κO)L^n-]^(n-1) released NO three times faster than cis-[Ru(bpy)₂(NO₂-κN)L^n-]^(n-1). We investigated endothelium-dependent vasodilation induced by cis-[Ru(bpy)₂(4-pic)(NO₂-κN)]⁺ and cis-[Ru(bpy)₂(4-pic)(NO₂-κO)]⁺ (4-pic = 4-picoline) in isolated 3 mm aortic rings precontracted with L-phenylephrine. Maximum vasodilation was achieved under 447 nm laser irradiation of 0.5 μMol.L^-1 ruthenium complexes for 100 s.

Keywords: Metal complexes; Nitric oxide; Nitro ruthenium (II); Nitro ruthenium-bipyridine; Photorelease; Vasodilation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Isomerism
Ligands
Nitric Oxide
Nitrogen Dioxide
Ruthenium* / chemistry
Ruthenium* / pharmacology
Vasodilator Agents*

Substances

Vasodilator Agents
Ruthenium
Nitric Oxide
Ligands
Nitrogen Dioxide