Alveolar Macrophages-Mediated Translocation of Intratracheally Delivered Perfluorocarbon Nanoparticles to Achieve Lung Cancer 19F-MR Imaging

Hongbin Wang; Xiaona Li; Jing Wang; Jiannan Wang; Hongyan Zou; Xuesong Hu; Linqing Yang; Penghui Shen; Rong A; Kaiqi Wang; Yingbo Li; Jie Yang; Kai Wang; Lili Yang; Lina Wu; Xilin Sun

doi:10.1021/acs.nanolett.3c00354

Alveolar Macrophages-Mediated Translocation of Intratracheally Delivered Perfluorocarbon Nanoparticles to Achieve Lung Cancer ¹⁹F-MR Imaging

Nano Lett. 2023 Apr 12;23(7):2964-2973. doi: 10.1021/acs.nanolett.3c00354. Epub 2023 Mar 22.

Authors

Hongbin Wang^{1

2}, Xiaona Li^{1

2}, Jing Wang^{1

2}, Jiannan Wang^{1

2}, Hongyan Zou^{1

2}, Xuesong Hu^{1

2}, Linqing Yang^{1

2}, Penghui Shen^{1

2}, Rong A^{1

2}, Kaiqi Wang^{1

2}, Yingbo Li^{1

2}, Jie Yang^{1

2}, Kai Wang^{1

2}, Lili Yang^{1

2}, Lina Wu^{1

2}, Xilin Sun^{1

2}

Affiliations

¹ Department of Nuclear Medicine, The Fourth Hospital of Harbin Medical University, Harbin 150028, China.
² NHC Key Laboratory of Molecular Probe and Targeted Diagnosis and Therapy, Molecular Imaging Research Center (MIRC), Harbin Medical University, Harbin 150028, China.

PMID: 36947431
DOI: 10.1021/acs.nanolett.3c00354

Abstract

Recent advances in intratracheal delivery strategies have sparked considerable biomedical interest in developing this promising approach for lung cancer diagnosis and treatment. However, there are very few relevant studies on the behavior and mechanism of imaging nanoparticles (NPs) after intratracheal delivery. Here, we found that nanosized perfluoro-15-crown-5-ether (PFCE NPs, ∼200 nm) exhibite significant ¹⁹F-MRI signal-to-noise ratio (SNR) enhancement than perfluorooctyl bromide (PFOB NPs) up to day 7 after intratracheal delivery. Alveolar macrophages (AMs) engulf PFCE NPs, become PFCE NPs-laden AMs, and then migrate into the tumor margin, resulting in increased tumor PFCE concentration and ¹⁹F-MRI signals. AMs-mediated translocation of PFCE NPs to lung draning lymph nodes (dLNs) decreases the background PFCE concentration. Our results shed light on the dynamic AMs-mediated translocation of intratracheally delivered PFC NPs for effective lung tumor visualization and reveal a pathway to develop and promote the clinical translation of an intratracheal delivery-based imaging strategy.

Keywords: 19F-MRI; alveolar macrophages; lung cancer; nanoparticles; perfluorocarbon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorocarbons*
Humans
Lung Neoplasms* / drug therapy
Macrophages, Alveolar
Magnetic Resonance Imaging / methods
Nanoparticles*

Substances

Fluorocarbons