Mosquito diversity (Diptera: Culicidae) and medical importance in four Cambodian forests

Antsa Rakotonirina; Pierre-Olivier Maquart; Claude Flamand; Chea Sokha; Sébastien Boyer

doi:10.1186/s13071-023-05729-w

Mosquito diversity (Diptera: Culicidae) and medical importance in four Cambodian forests

Parasit Vectors. 2023 Mar 21;16(1):110. doi: 10.1186/s13071-023-05729-w.

Authors

Antsa Rakotonirina¹, Pierre-Olivier Maquart², Claude Flamand^{3

4}, Chea Sokha⁵, Sébastien Boyer^{2

6}

Affiliations

¹ Medical and Veterinary Entomology Unit, Institut Pasteur du Cambodge, 983, Phnom Penh, Cambodia. arakotonirina@pasteur-kh.org.
² Medical and Veterinary Entomology Unit, Institut Pasteur du Cambodge, 983, Phnom Penh, Cambodia.
³ Epidemiology Unit, Institut Pasteur du Cambodge, 983, Phnom Penh, Cambodia.
⁴ Mathematical Modeling of Infectious Diseases Unit, Institut Pasteur, Université Paris Cité, UMR2000, CNRS, Paris, France.
⁵ Wildlife Health Program, Wildlife Conservation Society, Phnom Penh, Cambodia.
⁶ Ecology & Emergence of Arthropod-Borne Pathogens Unit, Department of Global Health, Institut Pasteur, CNRS UMR2000, Paris, France.

Abstract

Background: A total of 290 mosquito species are recorded in Cambodia among which 43 are known vectors of pathogens. As Cambodia is heavily affected by deforestation, a potential change in the dynamic of vector-borne diseases (VDBs) could occur through alteration of the diversity and density of sylvatic vector mosquitoes and induce an increase in their interactions with humans. Understanding mosquito diversity is therefore critical, providing valuable data for risk assessments concerning the (re)emergence of local VBDs. Consequently, this study mainly aimed to understand the spatial and temporal distribution of sylvatic mosquito populations of Cambodia by determining which factors impact on their relative abundance and presence.

Methods: A study was conducted in 12 sites from four forests in Cambodia. All mosquitoes, collected during the dry and rainy seasons, were morphologically identified. The diversity and relative density of mosquito species in each site were calculated along with the influence of meteorological and geographical factors using a quasi-Poisson generalized linear model.

Results: A total of 9392 mosquitoes were collected belonging to 13 genera and 85 species. The most represented genera were Culex, accounting for 46% of collected mosquitoes, and Aedes (42%). Besides being the most abundant species, Culex pseudovishnui and Aedes albopictus, which are known vectors of numerous arboviruses, were present in all sites during both dry and rainy seasons. The presence of mosquito species reported to be zoo-anthropophilic feeders was also observed in both forested and urban areas. Finally, this study demonstrated that altitude, temperature and precipitation impacted the abundance of mosquitoes but also influenced species community composition.

Conclusion: The results indicate an important diversity of mosquitoes in the four forests and an influence of meteorological and geographical factors on their community. Additionally, this work highlights in parallel the abundance of species considered to be of medical importance and therefore underlines the high risk of pathogen emergence/re-emergence in the region.

Keywords: Cambodia; Entomology; Forest; Vector mosquitoes.

MeSH terms

Aedes*
Animals
Biodiversity
Cambodia
Culex*
Culicidae*
Forests
Humans
Mosquito Vectors