Antioxidant status in patients with bladder cancer regarding cancer stage and grade

Zahid Lepara; Jasmin Alić; Orhan Lepara; Hajrudin Spahović; Almir Fajkić

doi:10.1016/j.ajur.2021.12.008

Antioxidant status in patients with bladder cancer regarding cancer stage and grade

Asian J Urol. 2023 Apr;10(2):182-188. doi: 10.1016/j.ajur.2021.12.008. Epub 2022 Aug 3.

Authors

Zahid Lepara¹, Jasmin Alić¹, Orhan Lepara², Hajrudin Spahović¹, Almir Fajkić³

Affiliations

¹ Urology Clinic, Clinical Center University of Sarajevo, Bolnička 25, 71 000 Sarajevo, Bosnia and Herzegovina.
² Department of Physiology, Faculty of Medicine, University of Sarajevo, Čekaluša 90, 71 000 Sarajevo, Bosnia and Herzegovina.
³ Department of Pathophysiology, Faculty of Medicine, University of Sarajevo, Čekaluša 90, 71 000 Sarajevo, Bosnia and Herzegovina.

Abstract

Objective: The imbalance of antioxidants and pro-oxidants plays a crucial role in the carcinogenesis of bladder cancer (BC). This study aimed to evaluate serum antioxidant status in patients with BC and determine its potential use in the diagnosis and progression potential considerations following histopathological assessment.

Methods: A cross-sectional study included 90 patients with BC, divided into Ta, T1, and T2-T4 stage subgroups, and according to cancer progression potential, into low-grade (LG) and high-grade (HG) subgroups. The control group (CG) included 30 healthy volunteers. Antioxidant status was determined using the spectrophotometric method and standard laboratory tests.

Results: Serum superoxide dismutase activity was significantly higher in BC patients regarding cancer stage in comparison to the CG (p<0.001). Catalase activity was highest in T2-T4 subgroup and was significantly higher compared to the Ta (p<0.01) and T1 (p<0.05) subgroups. Serum albumin level was significantly lower in the BC group compared to the CG (p<0.001). In addition, it was significantly lower in T2-T4 subgroup compared to T1 and Ta subgroups (p<0.01). A significant negative correlation was found between tumor size and serum albumin level only (r=-0.386, p<0.01). Catalase activity was higher in HG subgroup (p=0.009), while bilirubin level was higher in LG subgroup (p=0.035). The optimal cut-off value of catalase activity in differentiating patients with LG and HG BC subgroups was ≥11.96 IU/L, and the specificity and sensitivity were 51.1% and 82.2%, respectively. Bilirubin level, for a calculated optimal cut-off value of ≥11.95 μmol/L, had a specificity of 44.1% and sensitivity of 80.0%.

Conclusion: More invasive stages of BC with greater progression potential are associated with an increase in enzymatic antioxidant activity and a decrease in non-enzymatic antioxidant capacity. It may suggest a possible role of antioxidants in the prediction and monitoring of illness trajectory.

Keywords: Antioxidant; Bladder cancer; Catalase; Serum albumin; Superoxide dismutase.