Simultaneous removal of nitrogen and arsenite by heterotrophic nitrification and aerobic denitrification bacterium Hydrogenophaga sp. H7

Xia Fan; Li Nie; Zhengjun Chen; Yongliang Zheng; Gejiao Wang; Kaixiang Shi

doi:10.3389/fmicb.2022.1103913

Simultaneous removal of nitrogen and arsenite by heterotrophic nitrification and aerobic denitrification bacterium Hydrogenophaga sp. H7

Front Microbiol. 2023 Mar 3:13:1103913. doi: 10.3389/fmicb.2022.1103913. eCollection 2022.

Authors

Xia Fan^{1

2}, Li Nie¹, Zhengjun Chen¹, Yongliang Zheng², Gejiao Wang¹, Kaixiang Shi¹

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
² College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, China.

Abstract

Introduction: Nitrogen and arsenic contaminants often coexist in groundwater, and microbes show the potential for simultaneous removal of nitrogen and arsenic. Here, we reported that Hydrogenophaga sp. H7 was heterotrophic nitrification and aerobic denitrification (HNAD) and arsenite [As(III)] oxidation bacterium.

Methods: The appearance of nitrogen removal and As(III) oxidation of Hydrogenophaga sp. H7 in liquid culture medium was studied. The effect of carbon source, C/N ratio, temperature, pH values, and shaking speeds were analyzed. The impact of strains H7 treatment with FeCl3 on nitrogen and As(III) in wastewater was assessed. The key pathways that participate in simultaneous nitrogen removal and As(III) oxidation was analyzed by genome and proteomic analysis.

Results and discussion: Strain H7 presented efficient capacities for simultaneous NH₄ ⁺-N, NO₃ ^--N, or NO₂ ^--N removal with As(III) oxidation during aerobic cultivation. Strikingly, the bacterial ability to remove nitrogen and oxidize As(III) has remained high across a wide range of pH values, and shaking speeds, exceeding that of the most commonly reported HNAD bacteria. Additionally, the previous HNAD strains exhibited a high denitrification efficiency, but a suboptimal concentration of nitrogen remained in the wastewater. Here, strain H7 combined with FeCl3 efficiently removed 96.14% of NH₄ ⁺-N, 99.08% of NO₃ ^--N, and 94.68% of total nitrogen (TN), and it oxidized 100% of As(III), even at a low nitrogen concentration (35 mg/L). The residues in the wastewater still met the V of Surface Water Environmental Quality Standard of China after five continuous wastewater treatment cycles. Furthermore, genome and proteomic analyses led us to propose that the shortcut nitrification-denitrification pathway and As(III) oxidase AioBA are the key pathways that participate in simultaneous nitrogen removal and As(III) oxidation.

Keywords: Hydrogenophaga; arsenite oxidation; cocontamination; nitrogen removal; wastewater microbial treatment.